caa a22 4500 445795301 CHVBK 20180317145123.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1364-5 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1364-5 Melatonin Expresses Powerful Anti-inflammatory and Antioxidant Activities Resulting in Complete Improvement of Acetic-Acid-Induced Colitis in Rats [Elektronische Daten] [Gulgun Tahan, Roberto Gramignoli, Fabio Marongiu, Serdal Aktolga, Ali Cetinkaya, Veysel Tahan, Kenneth Dorko] Introduction: Increased free-radical production, decreased antioxidant capacity, and excessive inflammation are well-known features in the pathogenesis of inflammatory bowel disease. Melatonin is a powerful antioxidant and a scavenger of hydroxyl radicals. Melatonin has also been shown to have anti-inflammatory activities in tissues. Our study objective is to investigate the effects of melatonin on tissue inflammatory activities using an ulcerative colitis (UC) model induced by acetic acid (AA) in rats. Methods: Wistar rats (n=32) were divided into four groups. AA-induced colitis was performed in two of the groups, while the other two groups were injected with saline intrarectally. One of the AA-induced colitis groups and one of the control groups were administered 100mg/kg/day melatonin intraperitoneally, and the pair groups were given saline. After 4days, colonic changes were evaluated biochemically by measuring proinflammatory cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6], myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) levels in tissue homogenates and by histopathological examination. Results: AA caused colonic mucosal injury, whereas melatonin suppressed these changes in the AA-induced colitis group (P<0.001). AA administration resulted in increased TNF-α, IL-1β, IL-6, MPO, and MDA levels, and decreased GSH and SOD levels, whereas melatonin administration reversed these effects (all P<0.001). Conclusions: The present study proposes that melatonin has a dual action as an effective anti-inflammatory and an antioxidant, and may be a hopeful therapeutic agent for UC. Springer Science+Business Media, LLC, 2010 Melatonin nationallicence Ulcerative colitis nationallicence Inflammatory bowel disease nationallicence Oxidative stress nationallicence Tumor necrosis factor nationallicence Cytokine nationallicence Tahan Gulgun Department of Human Resources, University of Pittsburgh, 5846 Hobart Street FL2, 15217, Pittsburgh, PA, USA aut Gramignoli Roberto Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut Marongiu Fabio Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut Aktolga Serdal Department of Internal Medicine, Marmara University School of Medicine, Istanbul, Turkey aut Cetinkaya Ali Department of Internal Medicine, Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey aut Tahan Veysel Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut Dorko Kenneth Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 715-720 0163-2116 56:3<715 2011 56 10620 https://doi.org/10.1007/s10620-010-1364-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1364-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tahan Gulgun Department of Human Resources, University of Pittsburgh, 5846 Hobart Street FL2, 15217, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Gramignoli Roberto Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Marongiu Fabio Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Aktolga Serdal Department of Internal Medicine, Marmara University School of Medicine, Istanbul, Turkey aut NATIONALLICENCE 700 1- Cetinkaya Ali Department of Internal Medicine, Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey aut NATIONALLICENCE 700 1- Tahan Veysel Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Dorko Kenneth Department of Pathology, University of Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 715-720 0163-2116 56:3<715 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer