caa a22 4500 445795328 CHVBK 20180317145123.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10620-010-1333-z doi (NATIONALLICENCE)springer-10.1007/s10620-010-1333-z Protective Effects of Black Cumin ( Nigella sativa ) Oil on TNBS-Induced Experimental Colitis in Rats [Elektronische Daten] [F. Isik, Tugba Tunali Akbay, A. Yarat, Z. Genc, R. Pisiriciler, E. Caliskan-Ak, S. Cetinel, A. Altıntas, G. Sener] Background: The pathogenesis and treatment of ulcerative colitis remain poorly understood. The aim of the present study is to investigate the effects of black cumin (Nigella sativa) oil on rats with colitis. Methods: Experimental colitis was induced with 1mL trinitrobenzene sulfonic acid (TNBS) in 40% ethanol by intracolonic administration with 8-cm-long cannula under ether anesthesia to rats in colitis group and colitis + black cumin oil group. Rats in the control group were given saline at the same volume by intracolonic administration. Black cumin oil (BCO, Origo "100% natural Black Cumin Seed Oil,” Turkey) was given to colitis+black cumin oil group by oral administration during 3days, 5min after colitis induction. Saline was given to control and colitis groups at the same volume by oral administration. At the end of the experiment, macroscopic lesions were scored and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde, and glutathione levels, collagen content, and tissue factor, superoxide dismutase, and myeloperoxidase activities. Tissues were also examined by histological and cytological analysis. Proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6], lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples. Results: We found that black cumin oil decreased the proinflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis. Conclusions: BCO, by preventing inflammatory status in the blood, partly protected colonic tissue against experimental ulcerative colitis. Springer Science+Business Media, LLC, 2010 Colitis nationallicence Nigella sativa nationallicence Inflammation nationallicence Tissue factor activity nationallicence Oxidant damage nationallicence Isik F. Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut Tunali Akbay Tugba Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut Yarat A. Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut Genc Z. Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut Pisiriciler R. Faculty of Dentistry, Department of Histology and Embryology, Marmara University, Istanbul, Turkey aut Caliskan-Ak E. Faculty of Dentistry, Department of Histology and Embryology, Marmara University, Istanbul, Turkey aut Cetinel S. Faculty of Medicine, Department of Histology and Embryology, Marmara University, Istanbul, Turkey aut Altıntas A. Faculty of Pharmacy, Department of Pharmacognosy, Anadolu University, Eskisehir, Turkey aut Sener G. Faculty of Pharmacy, Department of Pharmacology, Marmara University, Istanbul, Turkey aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 721-730 0163-2116 56:3<721 2011 56 10620 https://doi.org/10.1007/s10620-010-1333-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1333-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Isik F. Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut NATIONALLICENCE 700 1- Tunali Akbay Tugba Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut NATIONALLICENCE 700 1- Yarat A. Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut NATIONALLICENCE 700 1- Genc Z. Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey aut NATIONALLICENCE 700 1- Pisiriciler R. Faculty of Dentistry, Department of Histology and Embryology, Marmara University, Istanbul, Turkey aut NATIONALLICENCE 700 1- Caliskan-Ak E. Faculty of Dentistry, Department of Histology and Embryology, Marmara University, Istanbul, Turkey aut NATIONALLICENCE 700 1- Cetinel S. Faculty of Medicine, Department of Histology and Embryology, Marmara University, Istanbul, Turkey aut NATIONALLICENCE 700 1- Altıntas A. Faculty of Pharmacy, Department of Pharmacognosy, Anadolu University, Eskisehir, Turkey aut NATIONALLICENCE 700 1- Sener G. Faculty of Pharmacy, Department of Pharmacology, Marmara University, Istanbul, Turkey aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/3(2011-03-01), 721-730 0163-2116 56:3<721 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer