caa a22 4500 445795336 CHVBK 20180317145123.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1717-8 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1717-8 Enhanced Expression of CXCL13 in Human Helicobacter pylori -Associated Gastritis [Elektronische Daten] [Yujiro Nakashima, Hajime Isomoto, Kayoko Matsushima, Akira Yoshida, Toshiyuki Nakayama, Masaaki Nakayama, Junzo Hisatsune, Tatsuki Ichikawa, Fuminao Takeshima, Tomayoshi Hayashi, Kazuhiko Nakao, Toshiya Hirayama, Shigeru Kohno] Background and Aims: Chemokine CXC ligand 13 (CXCL13) and CXC receptor type 5 (CXCR5) are constitutively expressed in tertiary lymphoid follicles where the CXCL13/CXCR5 system regulates B lymphocytes homing. In this study, we sought to examine CXCL13 expression in the H. pylori-infected and -uninfected gastric mucosa and to elucidate the implication in the pathogenesis of HAG in humans. Methods: Using endoscopic biopsies taken from the gastric antrum of 29 subjects infected with Helicobacter pylori and 22 uninfected subjects, mucosal CXCL13 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: The CXCL13 expression levels were significantly more elevated in H. pylori-positive patients than uninfected ones. The CXCL13 expression levels correlated with the degree of chronic gastritis and bacterial colonization. Immunohistochemistry and in vitro infection assay showed that CXCL13 was not produced by the gastric epithelium, but the α-smooth muscle antigen expressing mesenchymal cells were the possible source of CXCL13 within H. pylori-infected gastric mucosa. CXCR5 immunostaining was seen in the CD20-positive lymphoid aggregates. Conclusions: The enhanced induction of CXCL13 may be involved in the pathogenesis of H. pylori-associated gastritis. Springer Science+Business Media, LLC, 2011 Chemokine CXC ligand 13 nationallicence CXC receptor type 5 nationallicence Helicobacter pylori nationallicence Chronic inflammation nationallicence α-Smooth muscle antigen nationallicence Nakashima Yujiro Second Department of Internal Medicine, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Isomoto Hajime Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Matsushima Kayoko Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Yoshida Akira Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Nakayama Toshiyuki Department of Pathology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Nakayama Masaaki Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8102, Nagasaki, Japan aut Hisatsune Junzo Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8102, Nagasaki, Japan aut Ichikawa Tatsuki Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Takeshima Fuminao Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Hayashi Tomayoshi Department of Pathology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Nakao Kazuhiko Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Hirayama Toshiya Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8102, Nagasaki, Japan aut Kohno Shigeru Second Department of Internal Medicine, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2887-2894 0163-2116 56:10<2887 2011 56 10620 https://doi.org/10.1007/s10620-011-1717-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1717-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakashima Yujiro Second Department of Internal Medicine, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Isomoto Hajime Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Matsushima Kayoko Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Yoshida Akira Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Nakayama Toshiyuki Department of Pathology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Nakayama Masaaki Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hisatsune Junzo Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Ichikawa Tatsuki Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Takeshima Fuminao Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hayashi Tomayoshi Department of Pathology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Nakao Kazuhiko Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hirayama Toshiya Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 700 1- Kohno Shigeru Second Department of Internal Medicine, Nagasaki University Hospital, Sakamoto 1-7-1, 852-8102, Nagasaki, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2887-2894 0163-2116 56:10<2887 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer