caa a22 4500 445795344 CHVBK 20180317145123.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1701-3 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1701-3 Helicobacter bilis Colonization Enhances Susceptibility to Typhlocolitis Following an Inflammatory Trigger [Elektronische Daten] [Zhiping Liu, Amanda Ramer-Tait, Abigail Henderson, Cumhur Demirkale, Dan Nettleton, Chong Wang, Jesse Hostetter, Albert Jergens, Michael Wannemuehler] Background: Aberrant mucosal immune responses to antigens of the resident microbiota are a significant cause of inflammatory bowel diseases (IBD), as are genetic and environmental factors. Previous work from our laboratory demonstrated that Helicobacter bilis colonization of immunocompetent, defined microbiota mice induced antigen-specific immune responses to the resident microbiota, yet these mice failed to develop colitis, suggesting that the immunological provocation induced by H. bilis alone was insufficient to induce disease. Aim: The purpose of this study was to test the hypothesis that the introduction of a bacterial provocateur such as H. bilis enhances the host's susceptibility to IBD following an inflammatory event. Methods: Defined microbiota (DM) mice colonized with H. bilis were administered low dose (1.5%) dextran sodium sulfate (DSS) in drinking water for 5days followed by a 4-day restitution period. Severity of lesions was assessed grossly and microscopically. Differential expression of select mucosal genes and histopathologic lesions was characterized. Results: Helicobacter bilis colonization increased the severity of intestinal inflammation induced by an inflammatory trigger in the form of low-dose DSS. An analysis of the molecular and cellular mechanisms associated with H. bilis colonization revealed significant increases in expression of mucosal genes associated with lymphocyte activation and inflammatory cell chemotaxis as well as increased infiltration of mucosal macrophages and T cells in mice colonized with H. bilis prior to DSS treatment versus DSS treatment alone. Conclusions: These results indicate that prior colonization with H. bilis heightens the host's sensitivity to enteric inflammation by altering mucosal homeostasis and initiating immune cell activation and migration. Springer Science+Business Media, LLC, 2011 Helicobacter bilis nationallicence Colitis nationallicence Immune cells nationallicence Chemotaxis nationallicence Activation nationallicence Liu Zhiping Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut Ramer-Tait Amanda Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut Henderson Abigail Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut Demirkale Cumhur Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA, USA aut Nettleton Dan Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA, USA aut Wang Chong Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA aut Hostetter Jesse Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA aut Jergens Albert Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA aut Wannemuehler Michael Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2838-2848 0163-2116 56:10<2838 2011 56 10620 https://doi.org/10.1007/s10620-011-1701-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1701-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Zhiping Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut NATIONALLICENCE 700 1- Ramer-Tait Amanda Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut NATIONALLICENCE 700 1- Henderson Abigail Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut NATIONALLICENCE 700 1- Demirkale Cumhur Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA, USA aut NATIONALLICENCE 700 1- Nettleton Dan Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA, USA aut NATIONALLICENCE 700 1- Wang Chong Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA aut NATIONALLICENCE 700 1- Hostetter Jesse Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA aut NATIONALLICENCE 700 1- Jergens Albert Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA aut NATIONALLICENCE 700 1- Wannemuehler Michael Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Room 2182, 50011-1250, Ames, IA, USA aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2838-2848 0163-2116 56:10<2838 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer