caa a22 4500 445795387 CHVBK 20180317145123.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1774-z doi (NATIONALLICENCE)springer-10.1007/s10620-011-1774-z Aberrant Methylation of Different DNA Repair Genes Demonstrates Distinct Prognostic Value for Esophageal Cancer [Elektronische Daten] [Zhi-Qiang Ling, Pei Li, Ming-Hua Ge, Fu-Jun Hu, Xian-Hua Fang, Zi-Min Dong, Wei-Min Mao] Background: DNA mismatch repair (MMR) deficiency results in a strong mutator phenotype and high-frequency microsatellite instability (MSI-H), which are the hallmarks of many tumors. Aim: The objective of this study is to investigate the promoter CpG island methylation status of mismatch repair genes human mutL homolog 1 (hMLH1), human mutS homolog 2 (hMSH2), and O6-methylguanine-DNA methyltransferase (MGMT) in esophageal squamous cell carcinoma (ESCC) and its roles in alkylating agents chemotherapy. Methods: Real-time methylation-specific polymerase chain reaction (PCR) (real-time MSP) was employed to detect promoter CpG island methylation of the hMLH1, hMSH2, as well as MGMT genes in 235 surgical tumor tissue samples from ESCC patients and their corresponding normal tissue samples. Results: Promoter CpG island methylation of hMLH1, hMSH2, and MGMT were detectable in 43.4, 28.9, and 40.4% of ESCC tumor DNA, respectively, and the loss rates of hMLH1, hMSH2, and MGMT protein expression were 48.6, 34.5, and 40.9% in tumor tissues, respectively. For the entire population of 235 ESCC patients who were enrolled in operating treatment combined with radiotherapy and chemotherapy with alkylating agents, there was a significant difference in the overall survival between patients with methylated MGMT promoter and those with an unmethylated MGMT promoter (P<0.05). Conclusion: Promoter CpG island methylation may be a frequent event in ESCC carcinogenesis. Detection of the methylated sequences of hMLH1, hMSH2, and MGMT appears to be promising as a predictive factor in primary ESCC. Springer Science+Business Media, LLC, 2011 Esophageal squamous cell carcinoma (ESCC) nationallicence Promoter CpG island hypermethylation nationallicence O6-methylguanine-DNA methyltransferase ( MGMT ) gene nationallicence Human mutL homolog 1 ( hMLH1 ) gene nationallicence Human mutS homolog 2 ( hMSH2 ) gene nationallicence Prognosis nationallicence Ling Zhi-Qiang Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut Li Pei Department of Pathophysiology, College of Medical Sciences, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Ge Ming-Hua Department of Tumor Surgery, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut Hu Fu-Jun Department of Radiotherapy, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut Fang Xian-Hua Department of Pathology, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut Dong Zi-Min Department of Pathophysiology, College of Medical Sciences, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Mao Wei-Min Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2992-3004 0163-2116 56:10<2992 2011 56 10620 https://doi.org/10.1007/s10620-011-1774-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1774-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ling Zhi-Qiang Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Pei Department of Pathophysiology, College of Medical Sciences, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ge Ming-Hua Department of Tumor Surgery, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Fu-Jun Department of Radiotherapy, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Fang Xian-Hua Department of Pathology, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Dong Zi-Min Department of Pathophysiology, College of Medical Sciences, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Mao Wei-Min Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, No. 38 Guangji Rd., Banshanqiao District, 310022, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2992-3004 0163-2116 56:10<2992 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer