caa a22 4500 445795468 CHVBK 20180317145123.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1697-8 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1697-8 NaOH-Induced Crohn's Colitis in Rats: A Novel Experimental Model [Elektronische Daten] [Erdem Koçak, Seyfettin Köklü, Erdem Akbal, Adnan Taş, Gökhan Karaca, Müzeyyen Astarcı, Berrak Güven, Murat Can] Aim: Several models of experimental ulcerative colitis have been previously reported. To date, only one model of Crohn's colitis that was induced by trinitrobenzene sulfonic acid has been described. Materials and Methods: Twenty-one male albino rats were divided into three groups: group I: sham control group (n=7), group II: acetic acid group (n=7), group III: NaOH group (n=7). On the day of induction, all rats were lightly anesthetized with intramuscular ketamine (8mg/kg). A 6F plastic catheter was inserted rectally until the tip was 5cm proximal to the anus. Then, 2ml of 0.9% saline, 2ml of 4% acetic acid, and 2ml of 6.25% NaOH was administered to groups I, II, and III, respectively. All rats were sacrificed 5days after colitis induction. The distal colon segment was assessed macroscopically and microscopically. In addition, malondialdehyde (MDA) and nitric oxide (NO) levels of the colonic tissue and changes in body weight were measured. Results: Macroscopic and microscopic examinations of colonic tissue samples showed morphological similarities to human Crohn's disease (CD). The MDA and NO levels of the colonic tissues were significantly higher in the NaOH group compared to the acetic acid and sham control groups (P=0.001). Conclusion: NaOH may be used to induce Crohn's colitis as an experimental model. Springer Science+Business Media, LLC, 2011 Inflammatory bowel disease nationallicence Crohn's disease nationallicence NaOH nationallicence Koçak Erdem Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut Köklü Seyfettin Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut Akbal Erdem Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut Taş Adnan Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut Karaca Gökhan Department of General Surgery, Ankara Education and Research Hospital, Ankara, Turkey aut Astarcı Müzeyyen Department of Pathology, Ankara Education and Research Hospital, Ankara, Turkey aut Güven Berrak Department of Biochemistry, Zonguldak Karaelmas University, Zonguldak, Turkey aut Can Murat Department of Biochemistry, Zonguldak Karaelmas University, Zonguldak, Turkey aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2833-2837 0163-2116 56:10<2833 2011 56 10620 https://doi.org/10.1007/s10620-011-1697-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1697-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koçak Erdem Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut NATIONALLICENCE 700 1- Köklü Seyfettin Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut NATIONALLICENCE 700 1- Akbal Erdem Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut NATIONALLICENCE 700 1- Taş Adnan Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey aut NATIONALLICENCE 700 1- Karaca Gökhan Department of General Surgery, Ankara Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Astarcı Müzeyyen Department of Pathology, Ankara Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Güven Berrak Department of Biochemistry, Zonguldak Karaelmas University, Zonguldak, Turkey aut NATIONALLICENCE 700 1- Can Murat Department of Biochemistry, Zonguldak Karaelmas University, Zonguldak, Turkey aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2833-2837 0163-2116 56:10<2833 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer