caa a22 4500 445795662 CHVBK 20180317145124.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1686-y doi (NATIONALLICENCE)springer-10.1007/s10620-011-1686-y Aberrant CpG Island Hypermethylation and Down-Regulation of Oct - 6 mRNA Expression in Human Hepatocellular Carcinoma [Elektronische Daten] [Jing-zhe Sun, Xue-xi Yang, Xiang-hong Li, Wei-wen Xu, Ying Wang, Wei Zhu, Ming Li] Background: Aberrant CpG island hypermethylation is a major epigenetic mechanism that can inactivate the transcription of cancer-related genes. Purpose: This study aimed to investigate whether Oct-6 transcription was regulated by CpG island methylation in hepatocellular carcinoma (HCC). Methods: Quantitative real-time PCR and the MassARRAY platform (Sequenom) were employed in 38 HCC tissues samples and four cell lines. Results: The levels of Oct-6 mRNA were decreased by more than twofold in 31 of 38 tumor tissues compared to that of adjacent non-cancerous tissues. Among the 31 tumor tissues with lower levels of Oct-6 mRNA, 17 tumor tissues also had higher methylation levels in Oct-6 CpG island. Based on these results, we hypothesized that CpG island hypermethylation may down-regulate Oct-6 mRNA expression in HCC. To confirm this hypothesis, we also analyzed the changes in Oct-6 mRNA expression and CpG island methylation in four HCC cell lines (Huh7, Bel-7402, HepG2 and SMMC-7721) after treatment with 0.1, 0.5 and 2.5μM 5-Aza-2-deoxycytidine (5-Aza-CdR), a demethylating agent. The results demonstrated that the CpG island methylation levels decreased and Oct-6 mRNA levels increased in a dose-dependent manner in both Huh7 and Bel7402 cells, but there were only slight changes in HepG2 cell. Interestingly, there were no significant alterations of Oct-6 mRNA levels observed in SMMC7721 cell; although lower levels of CpG island methylation were detected after treatment with 5-Aza-CdR. Conclusions: Our study shows that CpG island hypermethylation contributes to down-regulation of Oct-6 mRNA expression in HCC. Springer Science+Business Media, LLC, 2011 Hepatocellular carcinoma nationallicence Oct - 6 nationallicence Methylation nationallicence mRNA expression nationallicence Sun Jing-zhe School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut Yang Xue-xi School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut Li Xiang-hong Department of Hepatobiliary Surgery, Nan fang Hospital, 510515, Guangzhou, People's Republic of China aut Xu Wei-wen School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut Wang Ying Department of Research, Sun Yat-sen Cancer Center, 510515, Guangzhou, People's Republic of China aut Zhu Wei Guangzhou Center for Disease Control and Prevention, 510515, Guangzhou, People's Republic of China aut Li Ming School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 3072-3077 0163-2116 56:10<3072 2011 56 10620 https://doi.org/10.1007/s10620-011-1686-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1686-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Jing-zhe School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Xue-xi School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Xiang-hong Department of Hepatobiliary Surgery, Nan fang Hospital, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xu Wei-wen School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Ying Department of Research, Sun Yat-sen Cancer Center, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Wei Guangzhou Center for Disease Control and Prevention, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Ming School of Biotechnology, Southern Medical University, 510515, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 3072-3077 0163-2116 56:10<3072 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer