caa a22 4500 445795689 CHVBK 20180317145124.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1711-1 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1711-1 Epigenetic Silencing of MicroRNA-375 Regulates PDK1 Expression in Esophageal Cancer [Elektronische Daten] [Xiaoli Li, Rui Lin, Jiansheng Li] Background: MicroRNAs (miRNAs) are a class of small (19-25 nucleotides) noncoding RNAs that regulate the expressions of a wide variety of genes, including some involved in cancer development. Some recent studies show that DNA methylation contributes to down-regulation of microRNA-375 (miR-375) during tumorigenesis. Whether or not down-regulation of miR-375 also exists in esophageal cancer is unknown. Aim: Our aim was to test the hypothesis that down-regulation of miR-375 also exists in esophageal cancer. Methods: Expression of levels of miR-375 were examined using real-time PCR on paired esophageal cancer and adjacent non-cancerous tissues. The methylation status is detected by methylation specific-PCR (MSP). Results: The results show that miR-375 is downregulated by hypermethylation of the promoter in esophageal cancer tissues. Epigenetic silencing of miR-375 induced an up-regulation of its targets, 3-phosphoinositide-dependent protein kinase-1 (PDK1). Restoration of the miR-375 expression in esophageal cancer cell lines downregulated the PDK1 expression. Furthermore, the miR-375 expression was found to be inversely correlated with PDK1 expression in esophageal cancer. Conclusion: Thus, miR-375 is frequently down-regulated in esophageal cancer and is a negative regulator of PDK1. Springer Science+Business Media, LLC, 2011 miR-375 nationallicence Esophageal cancer nationallicence Hypermethylation nationallicence PDK1 nationallicence Li Xiaoli Department of Gerontology, The First Affiliated Hospital, Zhengzhou University, Institute of Clinical Medical Research of Universities in Henan Province, 1 East Jianshe Road, Zhengzhou, Henan Province, People's Republic of China aut Lin Rui Department of Gastroenterology, The First Affiliated Hospital, Zhengzhou University, 1 East Jianshe Road, 450000, Zhengzhou, Henan Province, People's Republic of China aut Li Jiansheng Department of Gastroenterology, The First Affiliated Hospital, Zhengzhou University, 1 East Jianshe Road, 450000, Zhengzhou, Henan Province, People's Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2849-2856 0163-2116 56:10<2849 2011 56 10620 https://doi.org/10.1007/s10620-011-1711-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1711-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiaoli Department of Gerontology, The First Affiliated Hospital, Zhengzhou University, Institute of Clinical Medical Research of Universities in Henan Province, 1 East Jianshe Road, Zhengzhou, Henan Province, People's Republic of China aut NATIONALLICENCE 700 1- Lin Rui Department of Gastroenterology, The First Affiliated Hospital, Zhengzhou University, 1 East Jianshe Road, 450000, Zhengzhou, Henan Province, People's Republic of China aut NATIONALLICENCE 700 1- Li Jiansheng Department of Gastroenterology, The First Affiliated Hospital, Zhengzhou University, 1 East Jianshe Road, 450000, Zhengzhou, Henan Province, People's Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2849-2856 0163-2116 56:10<2849 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer