caa a22 4500 445795743 CHVBK 20180317145124.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1689-8 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1689-8 The Protective Effect of the Recombinant 53-kDa Protein of Trichinella spiralis on Experimental Colitis in Mice [Elektronische Daten] [Linlin Du, Hao Tang, Zhongfu Ma, Jia Xu, Wenchao Gao, Jiajia Chen, Wenjia Gan, Zhaoping Zhang, Xinbing Yu, Xingwang Zhou, Xuchu Hu] Background: Helminth infection has been proven to reduce the severity of experimental inflammatory bowel disease (IBD). The excretory-secretory proteins of helminths play an important role in the process of immunomodulation. Aims: In the present study, we aimed to investigate the protective potential of recombinant Trichinella spiralis (TS) 53-kDa protein (rTsP53), a component of excretory-secretory proteins, on experimental colitis in mice. Methods: BALB/c mice were treated subcutaneously with 50μg rTsP53 three times at an interval of 5days. Colitis was induced by intrarectal administration of 5mg trinitrobenzene sulfonic acid (TNBS). Disease activities and macroscopic and microscopic scores were evaluated. To determine immune response provoked by rTsP53, we measured specific IgG1 and IgG2a values against rTsP53 in sera of mice. We also detected cytokine profiles as well as the markers of alternatively activated macrophages (M2) in mice. Results: RTsP53 ameliorated significantly the disease activity index (DAI) as well as the macroscopic and microscopic scores. IgG1 but not IgG2a was the predominant specific antibody detected in the sera of immunized mice, indicating the potential of stimulating T-helper (Th) 2 bias response by rTsP3. Pre-treatment with rTsP53 decreased serum Th1 cytokines (TNF-a, IFN-γ) and elevated serum levels of serum Th2 cytokines (IL-4, IL-13); it also decreased colonic Th1 cytokines (TNF-α, IL-6) and colonic regulatory cytokines (IL-10, TGF-β1). RTsP53 increased colonic M2 markers, arginase-1 (Arg-1), and found in inflammatory zone 1 (FIZZ1), compared to mice without rTsP53 pretreatment. Conclusions: RTsP53 is a potential protective agent for IBD. Springer Science+Business Media, LLC, 2011 Inflammatory bowel disease nationallicence 53-kDa protein nationallicence Trichinella spiralis nationallicence Alternatively activated macrophages nationallicence Th2 nationallicence Du Linlin Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut Tang Hao Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut Ma Zhongfu Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut Xu Jia Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut Gao Wenchao Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Chen Jiajia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Gan Wenjia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Zhang Zhaoping Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Zhou Xingwang Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Hu Xuchu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2810-2817 0163-2116 56:10<2810 2011 56 10620 https://doi.org/10.1007/s10620-011-1689-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1689-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Du Linlin Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Tang Hao Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Ma Zhongfu Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Xu Jia Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Gao Wenchao Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Chen Jiajia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Gan Wenjia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Zhang Zhaoping Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Zhou Xingwang Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Hu Xuchu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2810-2817 0163-2116 56:10<2810 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer