caa a22 4500 44579576X CHVBK 20180317145124.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10620-011-1699-6 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1699-6 Fecal Detection of Mycobacterium avium Paratuberculosis Using the IS900 DNA Sequence in Crohn's Disease and Ulcerative Colitis Patients and Healthy Subjects [Elektronische Daten] [Anna Tuci, Francesca Tonon, Lucia Castellani, Alessandro Sartini, Giulia Roda, Margherita Marocchi, Alessandra Caponi, Alessandra Munarini, Giancarlo Rosati, Giampaolo Ugolini, Lorenzo Fuccio, Michele Scagliarini, Franco Bazzoli, Andrea Belluzzi] Background and Aim: Despite the increasing evidence of MAP/DNA isolation in Crohn's disease (CD), its potential pathogenetic role remains unclear. To further clarify the possible relationship between MAP and CD, we investigated the presence of IS900 DNA fragment in feces from Crohn's disease and ulcerative colitis (UC) patients and from healthy controls (HC). Methods: Stool samples were collected from 31 CD, 20 UC, and 23 HC and stored at −20°C in 200-mg aliquots. DNA was extracted. MAP presence was detected with a specific PCR amplifying a 409-bp fragment from IS900. The specificity of PCR for IS900 was confirmed sequencing three positive products. Statistical analysis was performed using the Chi-square test. Results: Twenty-one of 31 CD (68%), 13 of 20 UC (65%) and 11 of 23 HC (48%) were MAP-positive (CD vs. HC: p=ns; UC vs. HC: p=ns). With the limits of a small sample size, the IS900-positive percentage in CD and UC was higher than HC, although the difference was not statistically significant. Conclusions: The possibility to track the MAP presence in human feces represents a new approach to the "MAP hypothesis”. Detection of MAP DNA in feces is very common, reaching very high prevalence both in CD and in UC and even in HC. Our findings seem consistent with a high prevalence of MAP asymptomatic infection among the general population and so the possible involvement of MAP in CD pathogenesis could be linked to a specific immune defective response. Springer Science+Business Media, LLC, 2011 Crohn's disease nationallicence Mycobacterium avium subsp. paratuberculosis nationallicence IS900 nationallicence PCR nationallicence Tuci Anna Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Tonon Francesca Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Castellani Lucia Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Sartini Alessandro Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Roda Giulia Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Marocchi Margherita Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Caponi Alessandra Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Munarini Alessandra Department of Internal Medicine, Endocrinology Unit and Centre for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Bologna, Italy aut Rosati Giancarlo Department of General Surgery Emergency and Organ Transplantation, University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy aut Ugolini Giampaolo Department of General Surgery Emergency and Organ Transplantation, University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy aut Fuccio Lorenzo Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Scagliarini Michele Department of Statistics, University of Bologna, Bologna, Italy aut Bazzoli Franco Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Belluzzi Andrea Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2957-2962 0163-2116 56:10<2957 2011 56 10620 https://doi.org/10.1007/s10620-011-1699-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1699-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tuci Anna Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Tonon Francesca Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Castellani Lucia Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Sartini Alessandro Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Roda Giulia Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Marocchi Margherita Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Caponi Alessandra Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Munarini Alessandra Department of Internal Medicine, Endocrinology Unit and Centre for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Bologna, Italy aut NATIONALLICENCE 700 1- Rosati Giancarlo Department of General Surgery Emergency and Organ Transplantation, University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy aut NATIONALLICENCE 700 1- Ugolini Giampaolo Department of General Surgery Emergency and Organ Transplantation, University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy aut NATIONALLICENCE 700 1- Fuccio Lorenzo Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Scagliarini Michele Department of Statistics, University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Bazzoli Franco Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 700 1- Belluzzi Andrea Department of Internal Medicine and Gastroenterology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138, Bologna, Italy aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/10(2011-10-01), 2957-2962 0163-2116 56:10<2957 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer