caa a22 4500 445795956 CHVBK 20180317145125.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10620-010-1401-4 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1401-4 Beneficial Effects of Follistatin in Hepatic Ischemia-Reperfusion Injuries in Rats [Elektronische Daten] [Mami Kanamoto, Mitsuo Shimada, Yuji Morine, Tomoharu Yoshizumi, Satoru Imura, Toru Ikegami, Hiroki Mori, Yusuke Arakawa] Background: Ischemia-reperfusion injury has been demonstrated in a variety of clinical settings. The morbidity associated with liver transplantation and major hepatic resections is partly a result of ischemia-reperfusion injury. Follistatin, an activin-binding protein, binds to activins and subsequently blocks their action. It was reported that blockade of the action of activin with administration of follistatin accelerates recovery from ischemia renal injury. This study was conducted to investigate the involvement of the activin-follistatin system in hepatic ischemia-reperfusion injury. Methods: Total hepatic ischemia for 30min was performed followed by reperfusion in a rat model. Rats were divided into two groups: a follistatin group and a control group. Follistatin (1μg/body), which is an activin-binding protein, was administered at the time of reperfusion. Results: Though 80% of animals survived in the follistatin group, four of five animals died in the control group within 3days after reperfusion (p<0.05). AST was significantly lower at 3h after reperfusion in the follistatin group (p<0.05). LDH was also lower at 6h after reperfusion in the follistatin group (p<0.05). Follistatin inhibited the mRNA expression of the βA subunit of activin. Moreover, the expression of IL-6, which is an inflammatory cytokine, was suppressed at 6h after reperfusion in the follistatin group (p<0.05). Conclusions: The present study demonstrated that treatment with follistatin reduced the expression of IL-6 and activin resulting in beneficial support for hepatic ischemia-reperfusion injuries. Springer Science+Business Media, LLC, 2010 Hepatic ischemia-reperfusion injury nationallicence Follistatin nationallicence Activin nationallicence Cytokine nationallicence Kanamoto Mami Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Shimada Mitsuo Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Morine Yuji Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Yoshizumi Tomoharu Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Imura Satoru Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Ikegami Toru Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Mori Hiroki Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Arakawa Yusuke Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1075-1081 0163-2116 56:4<1075 2011 56 10620 https://doi.org/10.1007/s10620-010-1401-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1401-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanamoto Mami Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Shimada Mitsuo Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Morine Yuji Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Yoshizumi Tomoharu Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Imura Satoru Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Ikegami Toru Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Mori Hiroki Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Arakawa Yusuke Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, 770-8503, Tokushima, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1075-1081 0163-2116 56:4<1075 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer