caa a22 4500 445795972 CHVBK 20180317145125.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10620-010-1399-7 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1399-7 Can an Immunohistochemistry Method Differentiate Intestinal Tuberculosis from Crohn's Disease in Biopsy Specimens? [Elektronische Daten] [Ali İnce, Pembegül Güneş, Ebubekir Şenateş, Mesut Sezikli, Arzu Tiftikçi, Oya Övünç] Background: It is sometimes difficult to diagnose whether a patient has intestinal tuberculosis or Crohn's disease because both have similar clinical, pathologic, and endoscopic features. However, their therapies are completely different and a mistake in diagnosis can result with deterioration. Many laboratory methods for the diagnosis of tuberculosis require considerable time to receive a diagnostic result. We wanted to evaluate whether an immunohistochemical tuberculosis staining method can be helpful for faster differentiation of biopsy materials. Methods: We used formalin-fixed paraffin-embedded histologically diagnosed small intestine (n=1), colon (n=7), skin (n=8), lung (n=5), lymph node (n=24) tuberculosis and Crohn's disease (n=28) biopsy materials only with granulomas. Demographic characteristics like age and gender were also obtained. Pathology specimens were stained immunohistochemically with an antibody to VP-M660, targeting the 38-kDa antigen of Mycobacterium tuberculosis. Results: In the M. tuberculosis group, 33/45 of patients have positive immunohistochemistry (IHC) staining (73% sensitivity, 93% specifity), whereas only two of 28 patients have positive staining in the Crohn's group (p<0.001). The positive staining with IHC was detected as 85.7, 75, 75, and 60% in colon, lymph node, skin, and lung granulomas, respectively, in M. tuberculosis patients. Conclusions: Immunohistochemical staining of biopsy specimens with anti-VP-M660 seems to be a simple and fast technique with 73% sensitivity and 93% specificity for establishing an earlier differentiation of M. tuberculosis from Crohn's disease. Springer Science+Business Media, LLC, 2010 Immunohistochemistry nationallicence Mycobacterium tuberculosis nationallicence Crohn's disease nationallicence Differential diagnosis nationallicence İnce Ali Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut Güneş Pembegül Pathology Division, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut Şenateş Ebubekir Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut Sezikli Mesut Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut Tiftikçi Arzu Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut Övünç Oya Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1165-1170 0163-2116 56:4<1165 2011 56 10620 https://doi.org/10.1007/s10620-010-1399-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1399-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- İnce Ali Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut NATIONALLICENCE 700 1- Güneş Pembegül Pathology Division, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut NATIONALLICENCE 700 1- Şenateş Ebubekir Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut NATIONALLICENCE 700 1- Sezikli Mesut Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut NATIONALLICENCE 700 1- Tiftikçi Arzu Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut NATIONALLICENCE 700 1- Övünç Oya Gastroenterology Clinic, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1165-1170 0163-2116 56:4<1165 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer