caa a22 4500 445796006 CHVBK 20180317145125.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10620-010-1375-2 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1375-2 The Expression of PTHLH in Human Gastric Mucosa Enterochromaffin-Like Cells [Elektronische Daten] [Cuiping Liu, Jianting Chen, Yu Guo, Lisheng Yang, Chengyi Zhao, Lan Bai] Background: Gastrectomy may disturb the body's mineral homeostasis, with osteopenia and osteoporosis being among the late outcomes. Parathyroid hormone-like hormone (PTHLH) was detected in rat gastric enterochromaffin-like (ECL) cells in 2005, and some researchers suggested that it was the hypothetical hormone gastrocalcin that is believed to lead to osteoporosis. Aims: Our objective was to learn whether PTHLH is expressed in human gastric ECL cells and to form a basic understanding about the relationship between PTHLH and gastrin. Methods: We collected normal human gastric mucosa specimens and serum samples from 28 patients. Results: RT-PCR and immunohistochemical analysis demonstrated weak expression of PTHLH in ECL cells at the RNA and protein levels. A low level of PTHLH expression was also found in the serum. Serum gastrin did have a significant positive correlation with the relative ratio of PTHLH mRNA to β-actin levels in gastric mucosa (r s=0.569, p=0.002). Conclusions: This indicates that PTHLH has a low signal expression in human gastric ECL cells and that serum gastrin levels correlate with PTHLH RNA levels in gastric mucosa. Further work is needed to evaluate the functional role of PTHLH in ECL cells and to determine whether PTHLH is gastrocalcin. The Author(s), 2010 PTHLH nationallicence Osteoporosis nationallicence ECL cells nationallicence Gastrocalcin nationallicence Liu Cuiping GuangDong Provincial Key Laboratory of Gastroenterology Department for Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut Chen Jianting Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut Guo Yu Department of Huiqiao Building, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut Yang Lisheng Department of Huiqiao Building, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut Zhao Chengyi Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut Bai Lan Department of Huiqiao Building, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 993-998 0163-2116 56:4<993 2011 56 10620 https://doi.org/10.1007/s10620-010-1375-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1375-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Cuiping GuangDong Provincial Key Laboratory of Gastroenterology Department for Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Chen Jianting Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Guo Yu Department of Huiqiao Building, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Yang Lisheng Department of Huiqiao Building, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Zhao Chengyi Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Bai Lan Department of Huiqiao Building, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 993-998 0163-2116 56:4<993 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer