caa a22 4500 445796022 CHVBK 20180317145125.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10620-010-1378-z doi (NATIONALLICENCE)springer-10.1007/s10620-010-1378-z 5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream [Elektronische Daten] [Caroline Caltabiano, Felipe Máximo, Ana Spadari, Daniel da Conceição Miranda, Marcia Serra, Marcelo Ribeiro, Carlos Martinez] Background: No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis. Aim: To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis. Methods: Twenty-six Wistar rats were divided into two groups corresponding to sacrifice at 2 or 4weeks after fecal diversion of the left colon by means of proximal colostomy and distal mucosa fistula. Each group was divided into two subgroups according to intervention in excluded colon performed with 0.9% saline solution or 5-ASA. Level of oxidative DNA damage was determined by comet assay in cells obtained from segments with and without fecal stream before and after H2O2 challenge. For statistical analysis, was used one-way analysis of variance (ANOVA), adopting a 5% significance level (P<0.05). Results: Levels of DNA damage were always higher in colon segments without fecal stream, regardless of the intervention solution employed. DNA damage in colon segments with and without fecal stream in animals irrigated with 5-ASA was lower when compared with those treated with saline solution, regardless of time of irrigation. These levels remained lower after intervention with 5-ASA, even after H2O2 challenge. Conclusions: Enema with 5-ASA reduces oxidative DNA damage in epithelial cells of colon segments without fecal stream, even after H2O2 challenge, confirming the effects of 5-ASA against DNA damage by oxygen free radicals. Springer Science+Business Media, LLC, 2010 Colitis nationallicence Mesalamine nationallicence 5-Aminosalicylic acid nationallicence Fatty acids nationallicence Volatile nationallicence Butyrates nationallicence Rats nationallicence Caltabiano Caroline Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut Máximo Felipe São Francisco University Medical Hospital, Bragança Paulista, SP, Brazil aut Spadari Ana São Francisco University Medical Hospital, Bragança Paulista, SP, Brazil aut da Conceição Miranda Daniel São Francisco University Medical School, Bragança Paulista, SP, Brazil aut Serra Marcia Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut Ribeiro Marcelo Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut Martinez Carlos Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1037-1046 0163-2116 56:4<1037 2011 56 10620 https://doi.org/10.1007/s10620-010-1378-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1378-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caltabiano Caroline Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 700 1- Máximo Felipe São Francisco University Medical Hospital, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 700 1- Spadari Ana São Francisco University Medical Hospital, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 700 1- da Conceição Miranda Daniel São Francisco University Medical School, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 700 1- Serra Marcia Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 700 1- Ribeiro Marcelo Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 700 1- Martinez Carlos Post-Graduate Program in Health Sciences, São Francisco University Medical School, Bragança Paulista, SP, Brazil aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1037-1046 0163-2116 56:4<1037 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer