caa a22 4500 445796170 CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10620-010-1398-8 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1398-8 Activated Syndecan-1 Shedding Contributes to Mice Colitis Induced by Dextran Sulfate Sodium [Elektronische Daten] [Xia Wang, Ye Chen, Yugang Song, Shaoheng Zhang, Xiaoyun Xie, Xianfei Wang] Background: Syndecan-1(Sdc1) plays important roles in many steps of inflammatory responses. In ulcerative colitis patients, decreased Sdc1 expression was observed and Sdc1 analogue heparin could improve the disease course. A better understanding of how Sdc1 functions in colitis will benefit the disease intervention. Aims: To evaluate the role of Sdc1 in dextran sulfate sodium (DSS)-induced colitis. Methods: BALB/c mice were grouped randomly into control, DSS, and heparin+DSS. The DSS group was given 4% DSS orally and heparin+DSS group was given 4% DSS with heparin (enoxaparin) subcutaneously, while the control was given distilled water orally. All mice were killed at day 7. Disease activities, histopathological changes, membrane-bound and free Sdc1 level and mRNA expression of Sdc1, IL-1, and IL-10 in colon mucosa were detected. Results: Significant colitis was observed in the DSS group, but disease activity index and histological score showed significant lower in the heparin+DSS group than those in the DSS group. Compared to the control group, decreased Sdc1 protein expression was detected in colon mucosa of DSS-induced colitis while Sdc1 ectodomain level in serum was much higher. Inhibited Sdc1 ectodomain shedding was detected in the heparin+DSS group compared to the DSS group. RT-PCR demonstrated that both IL-1 and IL-10 expression were up-regulated in DSS-induced colitis while heparin lessened the up-regulation extent. Conclusions: Sdc1 shedding is activated in DSS-induced colitis and heparin, which mimics Sdc1 functions, relieves colitis severity by inhibiting Sdc1 shedding and down-regulating cytokines expression. Springer Science+Business Media, LLC, 2010 Syndecan-1 nationallicence Heparin nationallicence Colitis nationallicence Heparan sulfate nationallicence Wang Xia Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Chen Ye Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Song Yugang Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Zhang Shaoheng Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Xie Xiaoyun Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Wang Xianfei Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1047-1056 0163-2116 56:4<1047 2011 56 10620 https://doi.org/10.1007/s10620-010-1398-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1398-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Xia Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Chen Ye Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Song Yugang Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Shaoheng Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Xie Xiaoyun Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Xianfei Guangdong Provincial Key Laboratory of Gastroenterology and Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1047-1056 0163-2116 56:4<1047 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer