caa a22 4500 445796200 CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10620-010-1394-z doi (NATIONALLICENCE)springer-10.1007/s10620-010-1394-z Impact of Obesity and Leptin on Protein Expression Profiles in Mouse Colon [Elektronische Daten] [Sara Padidar, Andrew Farquharson, Lynda Williams, Nigel Hoggard, Martin Reid, Gary Duncan, Janice Drew] Background: Elevated leptin levels in obesity are associated with increased risk of colon pathology, implicating leptin signaling in colon disease. However, leptin-regulated processes in the colon are currently uncharacterized. Previously, we demonstrated that leptin receptors are expressed on colon epithelium and that increased adiposity and elevated plasma leptin in rats are associated with perturbed metabolism in colon tissue. Thus, we hypothesize that obesity disrupts expression of proteins regulated by leptin in the colon. Methods: A proteomic analysis was conducted to investigate firstly, differences in the colon of mice lacking leptin and leptin signaling (ob/ob and db/db, respectively) by comparing protein expression profiles with wild-type mice. Secondly, responses to leptin challenge in wild-type mice and ob/ob mice were compared to identify leptin-regulated proteins and associated cellular processes. Results: Forty proteins were identified with significantly altered expression patterns associated with differences in leptin status in comparisons between all groups of mice. These proteins are associated with calcium binding, cell cycle, cell proliferation, electron transport chain, energy metabolism, protein folding and transport, redox regulation, structural proteins, and proteins involved in transport and regulation of mucus production. Conclusions: This study provides evidence that obesity and leptin significantly alter protein profiles of a number of proteins linked to cellular processes in colon tissues that may be linked to the increased risk of colon pathology associated with obesity. Springer Science+Business Media, LLC, 2010 Leptin receptor nationallicence Leptin signaling nationallicence Proteomics nationallicence Multiple protein expression forms nationallicence Colon cancer nationallicence 2DPAGE : Two-dimensional polyacrylamide gel electrophoresis nationallicence MPEFs : Multiple protein expression forms nationallicence LC/MS/MS : Liquid chromatography tandem mass spectrometry nationallicence ETC : Electron transport chain nationallicence Padidar Sara Molecular Nutrition Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Farquharson Andrew Molecular Nutrition Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Williams Lynda Metabolic Health Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Hoggard Nigel Metabolic Health Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Reid Martin Proteomic Unit, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Duncan Gary Proteomic Unit, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Drew Janice Molecular Nutrition Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1028-1036 0163-2116 56:4<1028 2011 56 10620 https://doi.org/10.1007/s10620-010-1394-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1394-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Padidar Sara Molecular Nutrition Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Farquharson Andrew Molecular Nutrition Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Williams Lynda Metabolic Health Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Hoggard Nigel Metabolic Health Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Reid Martin Proteomic Unit, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Duncan Gary Proteomic Unit, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Drew Janice Molecular Nutrition Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/4(2011-04-01), 1028-1036 0163-2116 56:4<1028 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer