caa a22 4500 445796251 CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1588-z doi (NATIONALLICENCE)springer-10.1007/s10620-011-1588-z Effects of Ischemia and Reperfusion on P2X2 Receptor Expressing Neurons of the Rat Ileum Enteric Nervous System [Elektronische Daten] [Ariane Paulino, Kelly Palombit, Gabriela Cavriani, Wothan Tavares-de-Lima, Márcia Mizuno, Aline Marosti, Marcos da Silva, Priscila Girotti, Edson Liberti, Patricia Castelucci] Purpose: We investigated the effects of ischemia/reperfusion in the intestine (I/R-i) on purine receptor P2X2-immunoreactive (IR) neurons of the rat ileum. Methods: The superior mesenteric artery was occluded for 45min with an atraumatic vascular clamp and animals were sacrificed 4h later. Neurons of the myenteric and submucosal plexuses were evaluated for immunoreactivity against the P2X2 receptor, nitric oxide synthase (NOS), choline acetyl transferase (ChAT), calbindin, and calretinin. Results: Following I/R-i, we observed a decrease in P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of neurons of the myenteric and submucosal plexuses. These studies also revealed an absence of calbindin-positive neurons in the I/R-i group. In addition, the colocalization of the P2X2 receptor with NOS, ChAT, and calretinin immunoreactivity in the myenteric plexus was decreased following I/R-i. Likewise, the colocalization between P2X2 and calretinin in neurons of the submucosal plexus was also reduced. In the I/R-i group, there was a 55.8% decrease in the density of neurons immunoreactive (IR) for the P2X2 receptor, a 26.4% reduction in NOS-IR neuron, a 25% reduction in ChAT-IR neuron, and a 47% reduction in calretinin-IR neuron. The density of P2X2 receptor and calretinin-IR neurons also decreased in the submucosal plexus of the I/R-i group. In the myenteric plexus, P2X2-IR, NOS-IR, ChAT-IR and calretinin-IR neurons were reduced in size by 50%, 49.7%, 42%, and 33%, respectively, in the I/R-i group; in the submucosal plexus, P2X2-IR and calretinin-IR neurons were reduced in size by 56% and 72.6%, respectively. Conclusions: These data demonstrate that ischemia/reperfusion of the intestine affects the expression of the P2X2 receptor in neurons of the myenteric and submucosal plexus, as well as density and size of neurons in this population. Our findings indicate that I/R-i induces changes in P2X2-IR enteric neurons that could result in alterations in intestinal motility. Springer Science+Business Media, LLC, 2011 Chemical coding nationallicence Myenteric neurons nationallicence Submucosal neurons nationallicence Ischemia and reperfusion, P2X2 receptor nationallicence Paulino Ariane Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Palombit Kelly Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Cavriani Gabriela Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Tavares-de-Lima Wothan Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Mizuno Márcia Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Marosti Aline Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut da Silva Marcos Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Girotti Priscila Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Liberti Edson Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Castelucci Patricia Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2262-2275 0163-2116 56:8<2262 2011 56 10620 https://doi.org/10.1007/s10620-011-1588-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1588-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paulino Ariane Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Palombit Kelly Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Cavriani Gabriela Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Tavares-de-Lima Wothan Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Mizuno Márcia Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Marosti Aline Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- da Silva Marcos Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Girotti Priscila Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Liberti Edson Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 700 1- Castelucci Patricia Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Dr. Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2262-2275 0163-2116 56:8<2262 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer