caa a22 4500 445796332 CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1595-0 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1595-0 Intragastrically Administered Lysophosphatidic Acids Protect Against Gastric Ulcer in Rats Under Water-Immersion Restraint Stress [Elektronische Daten] [Mika Adachi, Gou Horiuchi, Natsuki Ikematsu, Tamotsu Tanaka, Junji Terao, Kiyoshi Satouchi, Akira Tokumura] Background and Aim: Lysophosphatidic acid exerts important physiological effects on many types of animal cells through its specific binding to several G protein-coupled receptors. In particular, its potent wound-healing effect has attracted much attention. To determine whether lysophosphatidic acids in a foodstuff and Chinese medicine are effective in protecting against gastric ulcer, we subjected rats to water-immersion restraint stress. Methods and Results: Three direct administrations of a solution of lysophosphatidic acid with a C18 fatty acyl group to the rat stomach in a concentration range of 0.001-0.1mM resulted in a significant reduction in the number of gastric ulcers induced during water-immersion restraint stress, and the potencies were as follows: linoleoyl species=α-linolenoyl species>oleoyl species. Intragastric administrations of a solution of highly purified lysophosphatidic acid from soybean lecithin significantly protected against the stress-induced gastric ulcers at lower concentrations than partially purified lysophosphatidic acid from soybean lecithin did. In addition, administration of a decocted solution of antyu-san, and lysophosphatidic acid-rich Chinese medicine, to the stomach was more effective in protecting against stress-induced ulcer than decoctations of antyu-san lacking the corydalis tuber component that is rich in lysophosphatidic acid. Conclusions: These results clearly show that lysophosphatidic acid is the effective component of soybean lecithin and antyu-san in protection against stress-induced gastric ulcer in the rat model, and suggest that daily intake of lysophosphatidic acid-rich foods or Chinese medicines may be beneficial for prevention of stress-induced gastric ulcer in human subjects. Springer Science+Business Media, LLC, 2011 Lysophosphatidic acid nationallicence Antyu-san nationallicence Soybean lecithin nationallicence Gastric ulcer nationallicence Water immersion restraint stress nationallicence Rat stomach nationallicence Adachi Mika Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, 770-8505, Tokushima, Japan aut Horiuchi Gou Faculty of Life Science and Biotechnology, Fukuyama University, 1 Gakuencho, 729-0292, Fukuyama, Japan aut Ikematsu Natsuki Faculty of Life Science and Biotechnology, Fukuyama University, 1 Gakuencho, 729-0292, Fukuyama, Japan aut Tanaka Tamotsu Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, 770-8505, Tokushima, Japan aut Terao Junji Department of Food Chemistry, Graduate School of Nutrition and Bioscience, Institute of Health Biosciences, University of Tokushima, 3-18 Kuramot-ocho, 770-8503, Tokushima, Japan aut Satouchi Kiyoshi Faculty of Life Science and Biotechnology, Fukuyama University, 1 Gakuencho, 729-0292, Fukuyama, Japan aut Tokumura Akira Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, 770-8505, Tokushima, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2252-2261 0163-2116 56:8<2252 2011 56 10620 https://doi.org/10.1007/s10620-011-1595-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1595-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adachi Mika Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 700 1- Horiuchi Gou Faculty of Life Science and Biotechnology, Fukuyama University, 1 Gakuencho, 729-0292, Fukuyama, Japan aut NATIONALLICENCE 700 1- Ikematsu Natsuki Faculty of Life Science and Biotechnology, Fukuyama University, 1 Gakuencho, 729-0292, Fukuyama, Japan aut NATIONALLICENCE 700 1- Tanaka Tamotsu Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 700 1- Terao Junji Department of Food Chemistry, Graduate School of Nutrition and Bioscience, Institute of Health Biosciences, University of Tokushima, 3-18 Kuramot-ocho, 770-8503, Tokushima, Japan aut NATIONALLICENCE 700 1- Satouchi Kiyoshi Faculty of Life Science and Biotechnology, Fukuyama University, 1 Gakuencho, 729-0292, Fukuyama, Japan aut NATIONALLICENCE 700 1- Tokumura Akira Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2252-2261 0163-2116 56:8<2252 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer