caa a22 4500 445796340 CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1586-1 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1586-1 Transient Stasis of Pancreatic Fluid Flow Together with Mild Injury of the Pancreatic Duct Cause Chronic Pancreatitis [Elektronische Daten] [Taizo Yamaguchi, Yasuyuki Kihara, Mitsuyoshi Yamamoto, Makoto Otsuki] Background: Little is known about the etiopathogenesis of chronic pancreatitis, due mainly to the lack of simple animal models suitable to study inflammatory and fibrogenetic processes in the pancreas. Aims: The purpose of this study was to examine whether transient congestion of pancreatic fluid flow alone or slight ductal injury alone is sufficient, or where both are required, to induce chronic pancreatic injury. Methods: Three different models of pancreatitis were tested in rats induced by retrograde intraductal infusion of 40μl/100g body weight of 0.01% agarose (group A), 40μl/100g body weight of 0.1% sodium taurocholate (group T), or a mixture of the two solutions (group M). Histological alterations of the pancreas were examined by hematoxylin-eosin staining, changes in type IV collagen structure were studied by immunostaining, and the gelatinolytic activity of latent and active matrix metalloproteinase-2 (MMP-2) was analyzed by zymography. Results: In group A and T rats, histological alterations of the pancreas and the gelatinolytic activity of MMP-2 returned to baseline levels by day 14, and immunoreactivity for type IV collagen appeared as continuous lines along the basement membrane. In group M rats, however, acinar damage, fibrosis and fatty degeneration were observed even on day 56, and type IV collagen was detected as discontinuous lines until day 56. MMP-2 was significantly elevated from day 5 to day 42. Conclusions: Co-existence of transient stasis of pancreatic fluid flow, together with mild damage to the pancreatic duct and acinar cells, exert synergistic effects on the development of persistent pancreatic injury with continuous disorganization of type IV collagen in the basement membrane of the ducts. Springer Science+Business Media, LLC, 2011 Synergistic effects nationallicence Fatty degeneration nationallicence Type IV collagen nationallicence MMP-2 nationallicence MMP : Matrix metalloproteinase nationallicence ECM : Extracellular matrix nationallicence NaTc : Sodium taurocholate nationallicence BM : Basement membrane nationallicence HE : Hematoxylin and eosin nationallicence PBS : Phosphate buffered saline nationallicence SDS : Sodium dodecyl sulfate nationallicence PAGE : Polyacrylamide gel electrophoresis nationallicence Yamaguchi Taizo Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut Kihara Yasuyuki Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut Yamamoto Mitsuyoshi Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut Otsuki Makoto Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2326-2332 0163-2116 56:8<2326 2011 56 10620 https://doi.org/10.1007/s10620-011-1586-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1586-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Taizo Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Kihara Yasuyuki Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Yamamoto Mitsuyoshi Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Otsuki Makoto Department of Gastroenterology and Metabolism, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2326-2332 0163-2116 56:8<2326 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer