caa a22 4500 445796383 CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1601-6 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1601-6 Differentiation-Inducing Activity of Hydroxycamptothecin on Cancer Stem-Like Cells Derived from Hepatocellular Carcinoma [Elektronische Daten] [Yi Zhang, Wen-Jie Song, Fu-Qin Zhang, Wei-Hui Liu, Ke-Feng Dou] Background: Hydroxycamptothecin (HCPT) is an anti-tumor agent that can induce differentiation in human cancer cells. Recent evidence indicates that side population (SP) cells possess characteristics of stem-like cells, and may be capable of initiating tumor growth. Aims: The present study investigated the differentiation of cancer stem-like cells derived from hepatocellular carcinoma. Methods and Results: Flow cytometry was used to isolated SP cells from HCC cell line (MHCC97 cells). These SP cells exhibit several stem-like cell characteristics that are distinct from the main population (MP) cells in vitro. After 3days of induction with a low concentration of HCPT, the SP cells lost their capacity to proliferate and invade, and their tumorigenicity declined. Based on real-time quantitative RT-PCR, we also found that the expression of hepatocyte-specific markers such as α-fetoprotein, albumin, hepatocyte nuclear factor-4 and miR-122 gradually changed during the differentiation of SP cells. Conclusions: Our data suggest that a low concentration of HCPT can induce hepatocyte-specific differentiation of cancer stem-like cells from MHCC97 cells, offering a possible therapeutic strategy for the treatment of human malignancies. Springer Science+Business Media, LLC, 2011 Cancer stem cells nationallicence Side population nationallicence Differentiation nationallicence Hydroxycamptothecin nationallicence Hepatocellular carcinoma nationallicence Zhang Yi Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut Song Wen-Jie Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut Zhang Fu-Qin Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut Liu Wei-Hui Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut Dou Ke-Feng Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2473-2481 0163-2116 56:8<2473 2011 56 10620 https://doi.org/10.1007/s10620-011-1601-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1601-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yi Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Song Wen-Jie Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Fu-Qin Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Liu Wei-Hui Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Dou Ke-Feng Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changlexi Road, 710032, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2473-2481 0163-2116 56:8<2473 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer