caa a22 4500 44579643X CHVBK 20180317145126.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1599-9 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1599-9 Correlation Analysis Between Gene Expression Profile of Rat Liver Tissues and High-Fat Emulsion-Induced Nonalcoholic Fatty Liver [Elektronische Daten] [Cunshuan Xu, Gaiping Wang, Yunpeng Hao, Jia Zhi, Lianxing Zhang, Cuifang Chang] Background: Nonalcoholic fatty liver disease (NAFLD) is caused by fat metabolism disorders and thereby abnormal or excessive accumulation of fat in hepatocytes, and characterized by steatosis, inflammation, fibrosis, apoptosis or necrosis. Aim: This study was carried out to explore the correlation between gene expression profiles of rat livers and the occurrence and progression of NAFLD at the transcriptional level. Methods: A rat model of nonalcoholic steatohepatitis (NASH) was established by feeding male rats with high-fat emulsion via gavage, and Rat Genome 230 2.0 Array was used to detect gene expression profiles of liver tissues obtained from male rats following 0, 2, 4, and 6weeks of high-fat emulsion feeding. Methods of bioinformatics and systems biology were applied to analyze the correlation between gene expression changes and physiological activities involved in NAFLD. Results: In total, 93 function-known genes, including 36 up-regulated and 57 down-regulated, differed significantly in expression compared to those of control rats, and 18 physiological activities were closely related to NAFLD. Especially, the activity of cell differentiation was decreased during the whole process of NAFLD, and the activities of inflammation response, stimulus response, cell migration and adhesion were attenuated in the second, fourth and sixth week, respectively. In the fourth and sixth weeks, lipid metabolism and cell apoptosis were augmented, and the former might be associated with the enhanced expression of plin, acsl6, scd2, elovl3, etc. Conclusion: These data provide useful information on the global gene expression changes due to high-fat emulsion feeding and bring important insights into the mechanisms of NAFLD. Springer Science+Business Media, LLC, 2011 NAFLD nationallicence NASH nationallicence Gene expression profile nationallicence Systems biology nationallicence Physiological activity nationallicence Xu Cunshuan College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut Wang Gaiping College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut Hao Yunpeng College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut Zhi Jia College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut Zhang Lianxing Key Laboratory for Cell Differentiation Regulation, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut Chang Cuifang Key Laboratory for Cell Differentiation Regulation, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2299-2308 0163-2116 56:8<2299 2011 56 10620 https://doi.org/10.1007/s10620-011-1599-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1599-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Cunshuan College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Wang Gaiping College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Hao Yunpeng College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Zhi Jia College of Life Sciences, Henan Normal University, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Zhang Lianxing Key Laboratory for Cell Differentiation Regulation, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Chang Cuifang Key Laboratory for Cell Differentiation Regulation, No. 46, Construction East Road, 453007, Xinxiang, Henan, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2299-2308 0163-2116 56:8<2299 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer