caa a22 4500 445796510 CHVBK 20180317145127.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1630-1 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1630-1 Rectal Administration of Lipopolysaccharide and Ovalbumin Ameliorates Acute Murine Colitis [Elektronische Daten] [Jong Im, Byong Ye, Jung Kim, Hyun Jung, In Song, Joo Kim] Background: Repeated challenges of lipopolysaccharide (LPS) could reduce the expression of proinflammatory cytokines in vitro, and oral administration of ovalbumin (OVA) induces mucosal tolerance in vivo. However, the effect of local administration of LPS and OVA on experimental colitis in vivo remains unknown. Aims: This study was performed to elucidate the effect of rectal administration of LPS and OVA on an acute murine colitis induced by dextran sulfate sodium (DSS). Methods: BALB/c mice were rectally administered LPS with or without OVA followed by 3% DSS. Colitis was assessed by disease activity index (DAI) including weight loss, stool consistency and rectal bleeding, and histopathology. Primary colon epithelial cells were isolated and the expression of Toll-like receptor 4 (TLR4) was examined using the Western blot analysis. IL-6, IFN-γ and IL-10 mRNA levels in colonic tissue were assessed using real-time RT-PCR. Results: LPS administration significantly attenuated the severity of acute DSS-induced colitis as assessed by DAI and histopathologic scoring compared with the control group. Combined treatment of LPS and OVA restored body weight loss and further ameliorated the severity of acute DSS colitis. LPS pretreatment regardless of OVA administration decreased TLR4 expression. LPS and OVA pretreatment reduced IL-6 and IFN-γ mRNA expression and increased IL-10 mRNA expression compared with controls. Conclusion: Rectal administration of LPS attenuated acute murine colitis, possibly through TLR4 down-regulation, and combined treatment of OVA additionally ameliorated colonic inflammation associated with up-regulation of IL-10. Springer Science+Business Media, LLC, 2011 Tolerance nationallicence Lipopolysaccharide nationallicence Ovalbumin nationallicence Dextran sulfate sodium nationallicence Colitis nationallicence Im Jong Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut Ye Byong Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Ulsan, Korea aut Kim Jung Department of Microbiology, Hanyang University College of Medicine, Seoul, Korea aut Jung Hyun Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut Song In Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut Kim Joo Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2292-2298 0163-2116 56:8<2292 2011 56 10620 https://doi.org/10.1007/s10620-011-1630-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1630-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Im Jong Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut NATIONALLICENCE 700 1- Ye Byong Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Ulsan, Korea aut NATIONALLICENCE 700 1- Kim Jung Department of Microbiology, Hanyang University College of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Jung Hyun Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut NATIONALLICENCE 700 1- Song In Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Joo Department of Internal Medicine Liver Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-744, Seoul, Korea aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2292-2298 0163-2116 56:8<2292 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer