caa a22 4500 445796642 CHVBK 20180317145127.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10620-011-1626-x doi (NATIONALLICENCE)springer-10.1007/s10620-011-1626-x Acute Pancreatitis in Obesity: Adipokines and Dietary Fish Oil [Elektronische Daten] [Hayder Al-Azzawi, Terence Wade, Deborah Swartz-Basile, Sue Wang, Henry Pitt, Nicholas Zyromski] Background: Acute pancreatitis is a substantial clinical problem accounting for 240,000 hospital admissions yearly in the United States. Obesity is epidemic and is clearly an independent risk factor for increased severity of acute pancreatitis (AP). Adipose tissue is an endocrine organ that secretes a variety of metabolically active substances termed adipokines. However, the role of adipokines in modulating acute pancreatitis severity remains incompletely understood. Dietary fish oil is rich in omega-3 free fatty acids and attenuates adipose tissue-induced inflammation. Therefore, we hypothesized that feeding obese mice diets rich in fish oil would alter the adipokine milieu and attenuate the severity of pancreatitis. Methods: Lean (C57BL/6J) and obese (LepDb) mice were fed either a soybean oil- or fish oil-rich diet for 4weeks. AP was induced by six hourly intraperitoneal injections of cerulein (50μg/kg). Serum adipokine levels were measured, and pancreatitis severity was assessed histologically and by measuring pancreatic concentrations of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), myleoperoxidase (MPO), and monocyte chemoattractant protein-1 (MCP-1). Results: Obese mice developed more severe pancreatitis than lean mice. Fish oil significantly decreased serum leptin (lean and obese) and increased serum adiponectin (lean only). Fish oil did not alter the baseline pancreatic inflammatory milieu, nor did it change histologic or biochemical pancreatitis severity. Conclusion: These data demonstrate that a diet rich in fish oil altered the adipokine milieu in lean and congenitally obese mice; however, fish oil did not improve pancreatitis severity induced with cerulein hyperstimulation. Springer Science+Business Media, LLC, 2011 Adiponectin nationallicence Pancreatitis nationallicence Obesity nationallicence Omega-3 fatty acids nationallicence Al-Azzawi Hayder Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut Wade Terence Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut Swartz-Basile Deborah Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut Wang Sue Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut Pitt Henry Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut Zyromski Nicholas Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2318-2325 0163-2116 56:8<2318 2011 56 10620 https://doi.org/10.1007/s10620-011-1626-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1626-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Al-Azzawi Hayder Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Wade Terence Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Swartz-Basile Deborah Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Wang Sue Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Pitt Henry Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Zyromski Nicholas Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/8(2011-08-01), 2318-2325 0163-2116 56:8<2318 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer