caa a22 4500 44579674X CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1447-3 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1447-3 Small Intestinal Bacterial Overgrowth in Nonalcoholic Steatohepatitis: Association with Toll-Like Receptor 4 Expression and Plasma Levels of Interleukin 8 [Elektronische Daten] [Ahmed Shanab, Paul Scully, Orla Crosbie, Martin Buckley, Liam O'Mahony, Fergus Shanahan, Sanaa Gazareen, Eileen Murphy, Eamonn Quigley] Background: Experimental and clinical studies suggest an association between small intestinal bacterial overgrowth (SIBO) and nonalcoholic steatohepatitis (NASH). Liver injury and fibrosis could be related to exposure to bacterial products of intestinal origin and, most notably, endotoxin, including lipopolysaccharide (LPS). Aim: To compare the prevalence of SIBO and its relationships to LPS receptor levels and systemic cytokines in NASH patients and healthy control subjects. Methods: Eighteen NASH patients (eight males) and 16 age-matched and gender-matched healthy volunteers were studied. SIBO was assessed by the lactulose breath hydrogen test (LHBT), plasma lipopolysaccharide binding protein (LBP) levels by ELISA, and expression (as a percentage) of TLR-2 and 4 on CD14-positive cells by flow cytometry. Pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) were measured in plasma. Results: SIBO was more common in NASH patients than control subjects (77.78% vs. 31.25%; P<0.0001). LBP levels and TLR-2 expression were similar in both groups, TLR-4/MD-2 expression on CD14 positive cells was higher among NASH patients: expression, mean±SEM, NASH vs. control: 20.95±2.91% vs. 12.73±2.29%, P<0.05. Among the examined cytokines, only IL-8 levels were significantly higher in patients than control (P=0.04) and correlated positively with TLR-4 expression (r=0.5123, P=0.036). Conclusion: NASH patients have a higher prevalence of small intestinal bacterial overgrowth which is associated with enhanced expression of TLR-4 and release of IL-8. SIBO may have an important role in NASH through interactions with TLR-4 and induction of the pro-inflammatory cytokine, IL-8. Springer Science+Business Media, LLC, 2010 Nonalcoholic steatohepatitis nationallicence Small intestinal bacterial overgrowth nationallicence Microbiota nationallicence Lipopolysaccharide nationallicence Toll-like receptor 4 nationallicence Interleukin 8 nationallicence Shanab Ahmed Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut Scully Paul Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut Crosbie Orla Gastroenterology and Hepatology, Cork University Hospital, Cork, Ireland aut Buckley Martin Gastroenterology, Mercy University Hospital, Cork, Ireland aut O'Mahony Liam Swiss Institute for Allergy and Asthma Research, Ober Strasse 22, 7270, Davos, Switzerland aut Shanahan Fergus Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut Gazareen Sanaa General Medicine/Endocrinology, Faculty of Medicine, Menoufia University, Shebin El Kom, Egypt aut Murphy Eileen Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut Quigley Eamonn Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1524-1534 0163-2116 56:5<1524 2011 56 10620 https://doi.org/10.1007/s10620-010-1447-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1447-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shanab Ahmed Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut NATIONALLICENCE 700 1- Scully Paul Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut NATIONALLICENCE 700 1- Crosbie Orla Gastroenterology and Hepatology, Cork University Hospital, Cork, Ireland aut NATIONALLICENCE 700 1- Buckley Martin Gastroenterology, Mercy University Hospital, Cork, Ireland aut NATIONALLICENCE 700 1- O'Mahony Liam Swiss Institute for Allergy and Asthma Research, Ober Strasse 22, 7270, Davos, Switzerland aut NATIONALLICENCE 700 1- Shanahan Fergus Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut NATIONALLICENCE 700 1- Gazareen Sanaa General Medicine/Endocrinology, Faculty of Medicine, Menoufia University, Shebin El Kom, Egypt aut NATIONALLICENCE 700 1- Murphy Eileen Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut NATIONALLICENCE 700 1- Quigley Eamonn Alimentary Pharmabiotic Centre, Department of Medicine, University College Cork, National University of Ireland, Cork, Ireland aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1524-1534 0163-2116 56:5<1524 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer