caa a22 4500 445796812 CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1421-0 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1421-0 Sphingosine-1-Phosphate Regulates the Expression of Adherens Junction Protein E-Cadherin and Enhances Intestinal Epithelial Cell Barrier Function [Elektronische Daten] [Jose Greenspon, Ruiyun Li, Lan Xiao, Jaladanki Rao, Rex Sun, Eric Strauch, Terez Shea-Donohue, Jian-Ying Wang, Douglas Turner] Background: The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions. Methods: Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques. Results: S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell-cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER) in vitro. Conclusions: These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell-cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells. Springer Science+Business Media, LLC (outside the USA), 2010 Sphingosine nationallicence Intestinal barrier nationallicence Ca2+ signaling nationallicence Intestinal epithelium nationallicence Greenspon Jose Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA aut Li Ruiyun Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut Xiao Lan Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut Rao Jaladanki Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut Sun Rex Department of Gastroenterology, University of Maryland School of Medicine, Baltimore, MD, USA aut Strauch Eric Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA aut Shea-Donohue Terez Department of Gastroenterology, University of Maryland School of Medicine, Baltimore, MD, USA aut Wang Jian-Ying Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut Turner Douglas Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1342-1353 0163-2116 56:5<1342 2011 56 10620 https://doi.org/10.1007/s10620-010-1421-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1421-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Greenspon Jose Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Li Ruiyun Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Xiao Lan Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Rao Jaladanki Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Sun Rex Department of Gastroenterology, University of Maryland School of Medicine, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Strauch Eric Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Shea-Donohue Terez Department of Gastroenterology, University of Maryland School of Medicine, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Wang Jian-Ying Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Turner Douglas Department of Surgery, Baltimore Veterans Affairs Medical Center, 10 N. Greene Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1342-1353 0163-2116 56:5<1342 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer