caa a22 4500 445796855 CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1474-0 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1474-0 Mutational Analysis of the PTEN Gene and Its Effects in Esophageal Squamous Cell Carcinoma [Elektronische Daten] [Guiqin Hou, Zhaoming Lu, Mingyue Liu, Hongmin Liu, Lexun Xue] Background: Esophageal squamous cell carcinoma (ESCC) is one of the most frequently diagnosed cancers in China, but the etiology and mode of carcinogenesis of this disease remain poorly understood. The phosphatase and tensin homolog deleted from chromosome 10 (PTEN) with putative tumor suppressing is frequently mutated in many cancers. Aims: The aim of this study was to investigate whether there exists a mutation in the PTEN gene of the ESCC cells, and the effects of the wild type and mutated PTEN genes on the proliferation and apoptosis of the ESCC cells. Methods: The wild type and mutated PTEN genes were cloned from human placenta and ESCC cells, respectively, and their effects on the proliferation and apoptosis of the ESCC cells were investigated. Also, the relationship between the PTEN gene status and sensitivity of the EC9706 cells to cisplatin was determined in the xenografts of nude mice. Results: There were mutations in the PTEN gene from ESCC cells. The proliferation of the EC9706 cells was clearly inhibited by the wild type PTEN gene, but not by the mutated PTEN gene in vitro. Furthermore, the wild type PTEN gene inhibited the growth of transplantable tumor, induced cell apoptosis, and improved the sensitivity of the EC9706 cells to cisplatin in vivo. Conclusion: The findings of the present study demonstrate that there are mutations in the PTEN gene of the ESCC cells and that the wild type PTEN gene has important effects on the ESCC cells in vitro and in vivo. Springer Science+Business Media, LLC, 2010 Esophageal squamous cell carcinoma nationallicence PTEN gene nationallicence Mutation nationallicence Nude mice nationallicence Cisplatin nationallicence Hou Guiqin School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, 450001, Zhengzhou, Henan, China aut Lu Zhaoming Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, 450052, Zhengzhou, Henan, China aut Liu Mingyue People's Hospital of Henan Province, 450003, Zhengzhou, Henan, China aut Liu Hongmin School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, 450001, Zhengzhou, Henan, China aut Xue Lexun Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, 450052, Zhengzhou, Henan, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1315-1322 0163-2116 56:5<1315 2011 56 10620 https://doi.org/10.1007/s10620-010-1474-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1474-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hou Guiqin School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Lu Zhaoming Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, 450052, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Liu Mingyue People's Hospital of Henan Province, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Liu Hongmin School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Xue Lexun Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, 450052, Zhengzhou, Henan, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1315-1322 0163-2116 56:5<1315 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer