caa a22 4500 445796863 CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1467-z doi (NATIONALLICENCE)springer-10.1007/s10620-010-1467-z Altered Expression and Localization of Connexin32 in Human and Murine Gastric Carcinogenesis [Elektronische Daten] [Hyang Jee, Ki Nam, Hyo-Jung Kwon, Sang-Uk Han, Dae-Yong Kim] Background: Intercellular communication via gap junctions, composed of protein subunits called connexins (Cxs), plays a key role in controlling cell growth, differentiation and carcinogenesis. Impaired gap junctional intercellular communication has been reported in various cancers and diseases. Aims: We investigated Cx32 expression patterns and semiquantitatively assessed Cx32 expression in cancers and preneoplastic lesions. To determine if cell proliferation is correlated with Cx32 expression, we evaluated Ki67 expression in a gastric cancer mouse model. Methods: In human and mouse, normal stomach and gastric adenocarcinoma tissues were used for immunohistochemical analyses. Results: Cx32 was detected at cell-cell (intercellular) contact points in normal cells and exhibited punctate intercellular and intracytoplasmic staining in cancer cells. The frequency of Cx32 loss of expression was significantly higher in human adenocarcinomas than in normal stomach. As tumor cells were less differentiated, Cx32 expression levels and intercellular and intracytoplasmic staining were also significantly lower. The Cx32 expression pattern in the mouse gastric cancer model was similar in several important respects to that of human. In mucous metaplasia of the mouse stomach, Cx32 was mainly expressed in the cytoplasm of epithelial cells. There was also an inverse correlation between Cx32 expression and cell proliferation in mouse tumors. However, there was no difference in the levels of Cx32 mRNA between normal and cancerous tissues. Conclusions: These findings suggest that altered Cx32 expression, a loss of intercellular Cx32 and a gain of intracytoplasmic Cx32 in the form of punctate "dot”, plays an important role in the formation of gastric adenocarcinomas. Springer Science+Business Media, LLC, 2010 Connexin32 nationallicence Gastric adenocarcinoma nationallicence Immunohistochemistry nationallicence Tissue array nationallicence Cell proliferation nationallicence Jee Hyang Laboratory of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Korea aut Nam Ki Vanderbilt University School of Medicine, 37232-2733, Nashville, TN, USA aut Kwon Hyo-Jung Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Korea aut Han Sang-Uk Genomic Research Center for Gastroenterology, Ajou University School of Medicine, Suwon, Korea aut Kim Dae-Yong Laboratory of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Korea aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1323-1332 0163-2116 56:5<1323 2011 56 10620 https://doi.org/10.1007/s10620-010-1467-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1467-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jee Hyang Laboratory of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Nam Ki Vanderbilt University School of Medicine, 37232-2733, Nashville, TN, USA aut NATIONALLICENCE 700 1- Kwon Hyo-Jung Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Korea aut NATIONALLICENCE 700 1- Han Sang-Uk Genomic Research Center for Gastroenterology, Ajou University School of Medicine, Suwon, Korea aut NATIONALLICENCE 700 1- Kim Dae-Yong Laboratory of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1323-1332 0163-2116 56:5<1323 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer