caa a22 4500 44579688X CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1462-4 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1462-4 Pringle Maneuver Deteriorates Gut Barrier Dysfunction Induced by Extended-Liver Radiofrequency Ablation [Elektronische Daten] [Petros Ypsilantis, Maria Lambropoulou, Anastasia Grapsa, Ioannis Tentes, Christina Tsigalou, Maria Panopoulou, Constantinos Simopoulos] Background: Large volume radiofrequency ablation (RFA) of the liver disrupts intestinal mucosa barrier with subsequent bacterial translocation. Aims: To investigate the effect of the Pringle maneuver applied concurrently with extended liver RFA on gut barrier integrity and bacterial translocation. Materials and Methods: Rats were subjected to 30% liver RFA following laparotomy (group RFA), RFA plus 30min Pringle (group RFA+P), Pringle (group P) or sham operation (group S). Intestinal tissue specimens were excised for histopathological examination and assessment of mucosal morphometry, apoptotic activity, mitotic activity and oxidative state. Tissue specimens were collected from the mesenteric lymph nodes, non-ablated liver parenchyma, kidneys and lungs for bacterial culture. Blood samples were collected from the portal and systemic circulation for endotoxin level measurement. Results: In group RFA+P, intestinal histopathologic lesions, mucosal atrophy and crypt cell apoptosis were more prominent compared to group RFA. Mitotic activity was suppressed. Oxidative stress was equally induced in all experimental groups. The incidence of positive bacterial cultures, bacterial counts and endotoxin levels were higher in group RFA+P compared to the other groups. Conclusion: The application of the Pringle maneuver concurrently with extended liver RFA aggravates gut barrier dysfunction with more aggressive translocation of endotoxins and intestinal bacteria. Springer Science+Business Media, LLC, 2010 Radiofrequency ablation nationallicence Pringle nationallicence Gut barrier nationallicence Bacterial translocation nationallicence Ypsilantis Petros Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, University General Hospital of Alexandroupolis, 68100, Alexandroupolis, Greece aut Lambropoulou Maria Laboratory of Histology and Embryology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut Grapsa Anastasia Laboratory of Microbiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut Tentes Ioannis Laboratory of Biochemistry, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut Tsigalou Christina Laboratory of Biochemistry, University General Hospital of Alexandroupolis, Alexandroupolis, Greece aut Panopoulou Maria Laboratory of Microbiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut Simopoulos Constantinos Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, University General Hospital of Alexandroupolis, 68100, Alexandroupolis, Greece aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1548-1556 0163-2116 56:5<1548 2011 56 10620 https://doi.org/10.1007/s10620-010-1462-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1462-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ypsilantis Petros Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, University General Hospital of Alexandroupolis, 68100, Alexandroupolis, Greece aut NATIONALLICENCE 700 1- Lambropoulou Maria Laboratory of Histology and Embryology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut NATIONALLICENCE 700 1- Grapsa Anastasia Laboratory of Microbiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut NATIONALLICENCE 700 1- Tentes Ioannis Laboratory of Biochemistry, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut NATIONALLICENCE 700 1- Tsigalou Christina Laboratory of Biochemistry, University General Hospital of Alexandroupolis, Alexandroupolis, Greece aut NATIONALLICENCE 700 1- Panopoulou Maria Laboratory of Microbiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece aut NATIONALLICENCE 700 1- Simopoulos Constantinos Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, University General Hospital of Alexandroupolis, 68100, Alexandroupolis, Greece aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1548-1556 0163-2116 56:5<1548 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer