caa a22 4500 445796979 CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1426-8 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1426-8 The Neutrophil Respiratory Burst and Bacterial Digestion in Crohn's Disease [Elektronische Daten] [Bu'Hussain Hayee, Farooq Rahman, Jane Tempero, Sara McCartney, Stuart Bloom, Anthony Segal, Andrew Smith] Background: Neutrophils are a key part of the innate immune defence against microbes, using the respiratory burst (RB) to optimise killing and digestion. Previous studies of the neutrophil RB in Crohn's disease (CD) have yielded conflicting results. Methods: Superoxide production in response to phorbol-myristyl acetate (PMA) was measured in neutrophils from 100 patients with CD compared to 50 healthy controls (HCs) and 50 patients with ulcerative colitis (UC). A further 22 CD and 10 HCs were studied using f-Met-Leu-Phe (fMLP), and digestion of E. coli by neutrophils was also evaluated. Results: The mean±SEM PMA-stimulated RB (nmol O2/106 cells/min) was 10.86±0.26 in HCs, 9.76±0.23 in CD (P=0.02) and 10.04±0.28 in UC (P=0.09 vs HC and 0.47 vs CD). No significant effect of age, gender or medication was observed. The RB in three patients with presumed CD was found to be in the range expected in patients with inherited neutrophil disorders. Stimulation with fMLP was calcium dependent and attenuated in patients on 5-ASA. Digestion of E. coli by neutrophils was not different in HC vs CD (21.6 vs 20.53%, P=0.60). Conclusion: The significant reduction in neutrophil RB in CD does not appear to result in defective bacterial digestion and is therefore unlikely play a major role in pathogenesis. Three patients in this cohort of patients with presumed idiopathic CD were found to have a profound defect of the neutrophil RB. A high index of suspicion for such patients is prudent, as their prognosis can be improved by altering or augmenting the conventional treatment regimens employed for CD. Springer Science+Business Media, LLC, 2010 Neutrophil nationallicence Respiratory burst nationallicence Superoxide nationallicence Digestion nationallicence Crohn's disease nationallicence Hayee Bu'Hussain Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut Rahman Farooq Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut Tempero Jane Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut McCartney Sara Department of Gastroenterology, UCLH NHS Foundation Trust, NW1 2BU, London, UK aut Bloom Stuart Department of Gastroenterology, UCLH NHS Foundation Trust, NW1 2BU, London, UK aut Segal Anthony Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut Smith Andrew Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1482-1488 0163-2116 56:5<1482 2011 56 10620 https://doi.org/10.1007/s10620-010-1426-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1426-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayee Bu'Hussain Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut NATIONALLICENCE 700 1- Rahman Farooq Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut NATIONALLICENCE 700 1- Tempero Jane Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut NATIONALLICENCE 700 1- McCartney Sara Department of Gastroenterology, UCLH NHS Foundation Trust, NW1 2BU, London, UK aut NATIONALLICENCE 700 1- Bloom Stuart Department of Gastroenterology, UCLH NHS Foundation Trust, NW1 2BU, London, UK aut NATIONALLICENCE 700 1- Segal Anthony Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut NATIONALLICENCE 700 1- Smith Andrew Department of Molecular Medicine, University College London, WC1E 6JJ, London, UK aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1482-1488 0163-2116 56:5<1482 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer