caa a22 4500 445797029 CHVBK 20180317145128.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1458-0 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1458-0 Intercellular Space Volume Is Mainly Increased in the Basal Layer of Esophageal Squamous Epithelium in Patients with GERD [Elektronische Daten] [Helmut Neumann, Klaus Mönkemüller, Lucía Fry, Frank Dombrowski, Doerthe Kuester, Mike Beyer, Peter Malfertheiner] Background/Aims: At present, the dilation of esophageal intercellular spaces (ICS) is considered an early morphologic marker of acid damage in patients with GERD. Nevertheless, previous electron microscopic (EM) studies had focused only on the suprabasal layer of squamous epithelium or did not nearly specify which layer of squamous epithelium was studied. Therefore, we aimed to assess the volumetric amount of the ICS in all layers of SE in patients with GERD. Methods: In this study, 48 patients were prospectively included (NERD=18, ERD=17; Barrett′s esophagus=5, controls=8). All patients with ERD and NERD had typical reflux symptoms, as assessed by a valid GERD questionnaire. ICS volume was assessed by electron microscopy in the superficial, prickle cell, and basal layers of esophageal squamous epithelium using the method of Weibel. Results: ERD was associated with increased ICS volume in the basal layer (LA-A, p=0.038; LA-B, p=0.005) and prickle cell layer (LA-A, p=0.006; LA-B, p=0.007) as compared to controls. Comparisons between NERD and ERD patients revealed more dilated ICS in the basal layer (LA-B, p=0.007), prickle cell layer (LA-A, p=0.008; LA-B, p=0.001) and superficial layer (LA-B, p=0.018) in patients with ERD. Conclusions: Not only the diameter but also the volume of the ICS is increased in patients with GERD. Furthermore, the dilation of ICS is present in all three layers of the SE, being more pronounced in the basal layer. These findings support the concept that the impairment of the esophagus begins in the deeper parts of the esophageal epithelium. Springer Science+Business Media, LLC, 2010 Gastroesophageal reflux disease (GERD) nationallicence ERD nationallicence NERD nationallicence Barrett′s esophagus nationallicence Intestinal metaplasia nationallicence Intercellular spaces nationallicence DIS nationallicence Neumann Helmut Department of Medicine I, University of Erlangen-Nuremberg, Ulmenweg 18, 91054, Erlangen, Germany aut Mönkemüller Klaus Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut Fry Lucía Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut Dombrowski Frank Institute of Pathology, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut Kuester Doerthe Institute of Pathology, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut Beyer Mike Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut Malfertheiner Peter Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1404-1411 0163-2116 56:5<1404 2011 56 10620 https://doi.org/10.1007/s10620-010-1458-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1458-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neumann Helmut Department of Medicine I, University of Erlangen-Nuremberg, Ulmenweg 18, 91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Mönkemüller Klaus Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Fry Lucía Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Dombrowski Frank Institute of Pathology, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Kuester Doerthe Institute of Pathology, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Beyer Mike Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Malfertheiner Peter Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1404-1411 0163-2116 56:5<1404 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer