caa a22 4500 445797037 CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1432-x doi (NATIONALLICENCE)springer-10.1007/s10620-010-1432-x Protective Effects of Kurozu and Kurozu Moromimatsu on Dextran Sulfate Sodium-Induced Experimental Colitis [Elektronische Daten] [Toru Shizuma, Kazuo Ishiwata, Masanobu Nagano, Hidezo Mori, Naoto Fukuyama] Background: Kurozu, a traditional Japanese black vinegar made from unpolished rice, and Kurozu Moromimatsu (Kurozu-M), its sediment, are both consumed in Japan as health foods or supplements. However, it is not known whether they have anti-colitis activity. Aims: We examined the protective effects of Kurozu and Kurozu-M in an animal model of dextran sulfate sodium (DSS)-induced colitis. Methods: DSS-induced colitis was induced in C57 black 6 mice by orally administering 3.5% DSS solution for 12days. The control group received basal CE-2 diet (n=10), the Kurozu group received CE-2 containing Kurozu (n=10), the Kurozu-M group received CE-2 containing Kurozu-M (n=10), and the acetic acid group received CE-2 containing acetic acid (n=10), starting a week before DSS administration. Changes of body weight and bloody stool frequency were monitored. At 12days after DSS administration, mice were killed for pathological examination and measurement of nitrotyrosine levels in rectal tissues. Results: Kurozu significantly inhibited body weight loss during 6-12days after DSS administration and reduced bloody stool frequency during 2-12days, and also significantly decreased nitrotyrosine levels at 12days, compared to the control group. Kurozu-M significantly inhibited body weight loss during 6-8days after DSS administration and reduced bloody stool frequency during 2-12days, but tissue nitrotyrosine level was not significantly different from the control. Acetic acid had no ameliorating effect on DSS-induced colitis compared to the control group. Conclusions: Kurozu and Kurozu-M have protective effects against DSS-induced colitis. Kurozu has anti-oxidative and anti-nitration activity. Springer Science+Business Media, LLC, 2010 Kurozu nationallicence Kurozu Moromimatsu nationallicence Dextran sulfate sodium nationallicence Colitis nationallicence Nitrotyrosine nationallicence Shizuma Toru Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut Ishiwata Kazuo Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut Nagano Masanobu Sakamoto Kurozu Inc., Kagoshima, Japan aut Mori Hidezo Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut Fukuyama Naoto Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1387-1392 0163-2116 56:5<1387 2011 56 10620 https://doi.org/10.1007/s10620-010-1432-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1432-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shizuma Toru Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Ishiwata Kazuo Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Nagano Masanobu Sakamoto Kurozu Inc., Kagoshima, Japan aut NATIONALLICENCE 700 1- Mori Hidezo Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Fukuyama Naoto Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, 259-1193, Isehara, Kanagawa, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1387-1392 0163-2116 56:5<1387 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer