caa a22 4500 445797045 CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1461-5 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1461-5 Endogenous Hydrogen Sulfide Is an Anti-inflammatory Molecule in Dextran Sodium Sulfate-Induced Colitis in Mice [Elektronische Daten] [Ikuhiro Hirata, Yuji Naito, Tomohisa Takagi, Katsura Mizushima, Takahiro Suzuki, Tatsushi Omatsu, Osamu Handa, Hiroshi Ichikawa, Hideo Ueda, Toshikazu Yoshikawa] Background: Endogenous hydrogen sulfide (H2S) is increasingly being recognized as an important gaseous physiological mediator. Accumulating evidence shows the functions of H2S in various models of disease, but rarely in colitis. In this study, we investigated the role of endogenous H2S in a dextran sodium sulfate (DSS)-induced colitis model. Methods: Acute colitis was induced using 8% DSS in male BALB/c mice. The mRNA expression of cystathionine γ-lyase (CSE), the primary synthetase of H2S in the gastrointestinal tract, and cystathionine-β-synthetase (CBS) was measured by real-time RT-PCR. The amount of H2S in the colonic mucosa was measured by gas chromatography. Colitis severity was evaluated clinically, histologically, and biochemically under the condition of co-treatment with DL-propargylglycine (PAG), an irreversible CSE inhibitor, and sodium sulfide (Na2S), an H2S donor. Results: The mRNA expression levels of CSE and CBS, and the H2S content in the colonic mucosa were increased with time after DSS administration. The disease activity index, which was determined by weight loss, stool consistency, and intestinal bleeding, increased after DSS administration. PAG significantly enhanced the increase in the disease activity index scores. PAG also significantly increased tissue-associated myeloperoxidase activity and thiobarbituric acid-reactive substances in the inflamed mucosa. Moreover, Na2S counteracted these effects of PAG. Conclusions: Taken together, the results indicated that the inhibition of endogenous H2S generation caused the deterioration of DSS-induced colitis. We conclude that physiological H2S might act as an anti-inflammatory molecule in colitis. Springer Science+Business Media, LLC, 2010 Hydrogen sulfide nationallicence Dextran sodium sulfate nationallicence Inflammation nationallicence Colitis nationallicence Lipid peroxidation nationallicence Hirata Ikuhiro Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Naito Yuji Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Takagi Tomohisa Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Mizushima Katsura Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Suzuki Takahiro Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Omatsu Tatsushi Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Handa Osamu Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Ichikawa Hiroshi Department of Medical Life Systems, Doshisha University, Kyoto, Japan aut Ueda Hideo Mitleben R&D Associates, Osaka, Japan aut Yoshikawa Toshikazu Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1379-1386 0163-2116 56:5<1379 2011 56 10620 https://doi.org/10.1007/s10620-010-1461-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1461-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirata Ikuhiro Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Naito Yuji Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Takagi Tomohisa Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Mizushima Katsura Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Suzuki Takahiro Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Omatsu Tatsushi Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Handa Osamu Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Ichikawa Hiroshi Department of Medical Life Systems, Doshisha University, Kyoto, Japan aut NATIONALLICENCE 700 1- Ueda Hideo Mitleben R&D Associates, Osaka, Japan aut NATIONALLICENCE 700 1- Yoshikawa Toshikazu Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, 602-8566, Kamigyo-ku, Kyoto, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1379-1386 0163-2116 56:5<1379 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer