caa a22 4500 44579707X CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1428-6 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1428-6 Monophosphoryl Lipid A-Induced Delayed Preconditioning in Rat Small Intestine Is Mediated by Calcitonin Gene-Related Peptide [Elektronische Daten] [Cai-Hong Yang, Ming-Sheng Zhang, Jie Li, Xuan-Ping Zhang, Hang Wang, Yi-Bin Hao] Background: Protective effects of ischemic preconditioning in rat small intestine have been shown to be related to the release of calcitonin gene-related peptide. Aims: The purpose of the present study was to explore whether monophosphoryl lipid A participated in the protective process of the delayed ischemic preconditioning in the peripheral vascular bed (the anse intestinalis of rat), and whether endogenous calcitonin gene-related peptide is a mediator in this process. Methods: Intestinal ischemia was induced by occlusion of the superior mesenteric artery for 30min, followed by reperfusion for 60min. The intestinal lesions were evaluated by the measurement of serum lactate dehydrogenase, myeloperoxidase levels, and small intestine tissue contents of malondialdehyde. In addition, calcitonin gene-related peptide in plasma and superior mesenteric vein effluent were also examined. Results: Pretreatment with monophosphoryl lipid A (500μg/kg. i.p.) 24h prior to ischemia-reperfusion significantly alleviated the intestinal tissue histology lesions, decreased serum levels of lactate dehydrogenase, myeloperoxidase, and reduced tissue content of malondialdehyde. Moreover, monophosphoryl lipid A markedly increased plasma concentrations of calcitonin gene-related peptide. Pretreatment with capsaicin, which specifically depletes the neurotransmitter content of sensory nerves or calcitonin gene-related peptide-(8-37), a selective calcitonin gene-related peptide receptor antagonist, inhibited the increased calcitonin gene-related peptide release and subsequently abrogated the protection by monophosphoryl lipid A. Conclusions: In conclusion, monophosphoryl lipid A pharmacologically mimics delayed preconditioning and the protective effects are related to the stimulation of calcitonin gene-related peptide release in rat small intestine. Springer Science+Business Media, LLC, 2010 Monophosphoryl lipid A nationallicence Delayed preconditioning nationallicence CGRP (calcitonin gene-related peptide) nationallicence CGRP-(8-37) nationallicence Capsaicin nationallicence Rat small intestine nationallicence Yang Cai-Hong Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut Zhang Ming-Sheng Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut Li Jie Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut Zhang Xuan-Ping Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut Wang Hang National Human Genomics Research Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut Hao Yi-Bin Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1333-1341 0163-2116 56:5<1333 2011 56 10620 https://doi.org/10.1007/s10620-010-1428-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1428-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Cai-Hong Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Zhang Ming-Sheng Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Li Jie Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Zhang Xuan-Ping Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Wang Hang National Human Genomics Research Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Hao Yi-Bin Department of Pharmacology, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1333-1341 0163-2116 56:5<1333 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer