caa a22 4500 445797096 CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10620-010-1431-y doi (NATIONALLICENCE)springer-10.1007/s10620-010-1431-y Establishment of a Reflux Esophago-Laryngitis Model in Rats [Elektronische Daten] [Daisuke Asaoka, Akihito Nagahara, Masako Oguro, Hiroki Mori, Kosaburo Nakae, Yuko Izumi, Taro Osada, Mariko Hojo, Michiro Otaka, Sumio Watanabe] Background: To investigate the pathophysiology of reflux laryngitis, an experimental model is required. Aim: The aim of this study is to establish an animal model of reflux esophago-laryngitis, modifying our previously reported model of chronic acid reflux esophagitis. Methods: The modified chronic acid reflux esophagitis (m-RE) group (n=10), in which the duodenum was wrapped with 2.5mm of Nelaton catheter, was not treated with any drugs. Also postoperatively, two treatment groups (n=10 in each) received different dosages of rabeprazole (RPZ): 1.0mg/kg/day (RPZ1.0 group) or 10.0mg/kg/day (RPZ10.0 group). As a control group (n=5), other rats underwent sham operation. The esophagus and larynx were resected on day14 after the operation, and ulcer score of the esophagus was assessed. The epithelial thickness and leukocyte infiltration of the supraglottic and subglottic laryngeal mucosae were investigated. The number of interleukin (IL)-1β-positive cells was also counted and defined as the IL-1β labeling index. Results: In the m-RE group, the epithelial thickness, leukocyte infiltration, and IL-1β labeling index of the supraglottic and subglottic laryngeal mucosae were increased compared with controls (P<0.01). In the RPZ groups, not only the ulcer score of esophagus but also the epithelial thickness, leukocyte infiltration, and IL-1β labeling index of both the supraglottic and subglottic laryngeal mucosae were decreased dose-dependently relative to the m-RE group (P<0.05). Conclusions: Our modified chronic acid reflux esophagitis model proved useful in establishing a rat reflux esophago-laryngitis model, with both pathological laryngeal findings and reflux esophagitis shown to be improved by administration of a proton pump inhibitor. Springer Science+Business Media, LLC, 2010 Laryngopharyngeal reflux disease (LPRD) nationallicence Proton pump inhibitor nationallicence Reflux esophagitis nationallicence Reflux laryngitis nationallicence Asaoka Daisuke Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Nagahara Akihito Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Oguro Masako Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Mori Hiroki Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Nakae Kosaburo Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Izumi Yuko Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Osada Taro Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Hojo Mariko Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Otaka Michiro Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Watanabe Sumio Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1299-1308 0163-2116 56:5<1299 2011 56 10620 https://doi.org/10.1007/s10620-010-1431-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1431-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asaoka Daisuke Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Nagahara Akihito Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Oguro Masako Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Mori Hiroki Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakae Kosaburo Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Izumi Yuko Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Osada Taro Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Hojo Mariko Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Otaka Michiro Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 700 1- Watanabe Sumio Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/5(2011-05-01), 1299-1308 0163-2116 56:5<1299 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer