caa a22 4500 445797207 CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10620-010-1324-0 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1324-0 Gender- and Age-Related Differences in Muscular and Nerve-Mediated Responses in Human Colon [Elektronische Daten] [M. Maselli, P. Trisolini, I. Demma, F. Pezzolla, F. De Ponti] Background: Gender- and age-related differences in muscular and nerve-mediated responses in human colon are poorly characterized. We studied carbachol-induced motor responses and electrically evoked contractions in sigmoid circular muscle from adult and elderly patients of different gender. Methods: Sigmoid colon segments were obtained from 24 men and 16 women undergoing left hemicolectomy for colon cancer. Isometric tension was measured on muscle strips exposed to increasing carbachol concentrations. The effects of atropine, guanethidine, l-nitro arginine methyl ester (l-NAME), and tetrodotoxin on electrically evoked contractions were also studied. Results: Female patients showed higher maximal response to carbachol than male patients, elderly females being the most sensitive to carbachol among all patient groups. Electrically evoked contractions were linearly related to stimulation frequency and abolished by tetrodotoxin. Electrically evoked contractions were significantly more pronounced in elderly male patients; they were reduced by atropine and guanethidine and increased by l-nitro arginine methyl ester in the presence of atropine and guanethidine (P<0.05). The effect of l-NAME was most marked in elderly male patients and least pronounced in elderly females. Conclusions: The response to carbachol and the role of nitrergic pathways differ according to age and gender; this may depend on muscarinic receptor upregulation or humoral factors affecting nitric oxide release, respectively. Springer Science+Business Media, LLC, 2010 Gender nationallicence Age nationallicence Motility invitro nationallicence Human colon nationallicence Maselli M. Experimental Pharmacology Laboratory, Scientific Institute of Gastroenterology "S. de Bellis”, Via Turi, 27, 70013, Castellana Grotte, Italy aut Trisolini P. Experimental Pharmacology Laboratory, Scientific Institute of Gastroenterology "S. de Bellis”, Via Turi, 27, 70013, Castellana Grotte, Italy aut Demma I. Surgery Department, Scientific Institute of Gastroenterology "S. de Bellis”, Castellana Grotte, Bari, Italy aut Pezzolla F. Surgery Department, Scientific Institute of Gastroenterology "S. de Bellis”, Castellana Grotte, Bari, Italy aut De Ponti F. Department of Pharmacology, University of Bologna, Via Irnerio, 48, 40126, Bologna, Italy aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 352-358 0163-2116 56:2<352 2011 56 10620 https://doi.org/10.1007/s10620-010-1324-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1324-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maselli M. Experimental Pharmacology Laboratory, Scientific Institute of Gastroenterology "S. de Bellis”, Via Turi, 27, 70013, Castellana Grotte, Italy aut NATIONALLICENCE 700 1- Trisolini P. Experimental Pharmacology Laboratory, Scientific Institute of Gastroenterology "S. de Bellis”, Via Turi, 27, 70013, Castellana Grotte, Italy aut NATIONALLICENCE 700 1- Demma I. Surgery Department, Scientific Institute of Gastroenterology "S. de Bellis”, Castellana Grotte, Bari, Italy aut NATIONALLICENCE 700 1- Pezzolla F. Surgery Department, Scientific Institute of Gastroenterology "S. de Bellis”, Castellana Grotte, Bari, Italy aut NATIONALLICENCE 700 1- De Ponti F. Department of Pharmacology, University of Bologna, Via Irnerio, 48, 40126, Bologna, Italy aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 352-358 0163-2116 56:2<352 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer