caa a22 4500 445797304 CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10620-010-1299-x doi (NATIONALLICENCE)springer-10.1007/s10620-010-1299-x Protective Effect of Rebamipide Against Helicobacter pylori -CagA-Induced Effects on Gastric Epithelial Cells [Elektronische Daten] [Kyung Lee, Jee-Yeon Kim, Woo-Kyung Kim, Dong-Hun Shin, Kyung-Un Choi, Dae Kim, Woo-Jeong Lee, Jin-Hwa Choi, Soo Lee, Gwang Kim, Geun Song, Tae Jeon, Chi Kim, Ki Hong, Do Park] Background: Helicobacter pylori CagA dysregulates cell signaling pathways and leads to targeted transcriptional up-regulation of genes implicated in gastric cell injury. The aim of this study was to determine the effects of rebamipide on CagA-induced effects on gastric epithelial cells. We investigated the effects of rebamipide treatment (pre- or post-treatment before or after CagA transfection) on CagA-induced gastric cell injury. Method: We evaluated the morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization by confocal microscopy, IL-8 production by ELISA, and NF-κB activation by luciferase assay in AGS gastric epithelial cells and MDCK cells. Results: Transfection of CagA into gastric epithelial cells induced morphologic changes (hummingbird phenotype), ZO-1 mislocalization, and IL-8 production in gastric epithelial cells. Pre-treatment with rebamipide inhibits CagA-induced effects on gastric epithelial cells, including morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization, IL-8 production, and NF-κB activity. Conclusions: These results suggest that rebamipide might have a potential role in the protection of H. pylori CagA-induced effects on gastric epithelial cells. Springer Science+Business Media, LLC, 2010 Helicobacter pylori nationallicence CagA nationallicence IL-8 nationallicence ZO-1 nationallicence Rebamipide nationallicence Lee Kyung Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Kim Jee-Yeon Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Kim Woo-Kyung Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Shin Dong-Hun Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Choi Kyung-Un Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Kim Dae Department of Surgery, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut Lee Woo-Jeong Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Choi Jin-Hwa Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Lee Soo Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Kim Gwang Department of Internal Medicine, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut Song Geun Department of Internal Medicine, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut Jeon Tae Department of Surgery, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut Kim Chi Department of Pharmacology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut Hong Ki Department of Pharmacology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut Park Do Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 441-448 0163-2116 56:2<441 2011 56 10620 https://doi.org/10.1007/s10620-010-1299-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1299-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Kyung Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Jee-Yeon Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Woo-Kyung Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Shin Dong-Hun Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Choi Kyung-Un Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Dae Department of Surgery, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Lee Woo-Jeong Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Choi Jin-Hwa Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Lee Soo Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Gwang Department of Internal Medicine, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Song Geun Department of Internal Medicine, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Jeon Tae Department of Surgery, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Chi Department of Pharmacology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Hong Ki Department of Pharmacology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Park Do Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital and Medical Research Institute, Pusan National University, Ami-1 Ga, 602-739, Seo-Gu, Busan, Republic of Korea aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 441-448 0163-2116 56:2<441 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer