caa a22 4500 445797339 CHVBK 20180317145129.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10620-010-1302-6 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1302-6 Hemodynamic Assessment of the Development of Portal-Systemic Collaterals in Portal Hypertensive Rats [Elektronische Daten] [Che-Chang Chan, Shiow-Chwen Tsai, Ling-Yi Cheng, Fa-Yauh Lee, Han-Chieh Lin] Background: Portal hypertension induced the formation of portal-systemic collaterals. Revealing the developmental change of portal-systemic collaterals is important for future therapy. Methods: We observed the evolution of an accessible shunting vessel, the spleno-renal shunt (SRS), in rats after induction of portal hypertension by partial portal vein ligation (PVL). The hemodynamic and histological assessments of SRS were performed by transit time ultrasound and immunohistochemical studies. Results: The portal pressure of PVL rats surged to 16.5±1.1mmHg on day 1 after ligation surgery and was maintained at a significantly higher level (13.0±0.4mmHg) to day 14 when compared to sham rats (p<0.05). The size or flow of SRS in PVL rats did not change immediately after portal pressure surge. Instead, they increased rapidly on day 4, peaked on day 7, and stabilized thereafter. The size and flow were greater and the resistance of SRS was lower in PVL rats after day 7 (p<0.05). The anti-Ki67 immunohistochemical study demonstrated positive staining of endothelium in SRS and negative in portal vein or aorta of PVL rats. In addition, the endothelial cells of SRS were stained positive for CD31 and KLF5. Conclusions: We concluded that the pressure-induced opening of pre-existing vessels was not the primary underlying mechanism in the formation of SRS. Endothelial proliferating and vascular remodeling process participated actively during the development of SRS. These observations can be used for studying the pathogenesis and developing more effective anti-portal hypertensive therapy in the future. Springer Science+Business Media, LLC, 2010 Portal hypertension nationallicence Portal-systemic collaterals nationallicence Transit time ultrasound nationallicence Spleno-renal shunt nationallicence TTU : Transit time ultrasound nationallicence SRS : Spleno-renal shunt nationallicence Chan Che-Chang Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut Tsai Shiow-Chwen Taipei Physical Education College, Taipei, Taiwan, Republic of China aut Cheng Ling-Yi Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut Lee Fa-Yauh Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut Lin Han-Chieh Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 417-424 0163-2116 56:2<417 2011 56 10620 https://doi.org/10.1007/s10620-010-1302-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1302-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chan Che-Chang Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut NATIONALLICENCE 700 1- Tsai Shiow-Chwen Taipei Physical Education College, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Cheng Ling-Yi Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut NATIONALLICENCE 700 1- Lee Fa-Yauh Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut NATIONALLICENCE 700 1- Lin Han-Chieh Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec 2, Shih-Pai Road, Beitou District, Taipei city, Taiwan 112, Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 417-424 0163-2116 56:2<417 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer