caa a22 4500 445797487 CHVBK 20180317145130.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10620-010-1322-2 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1322-2 Protective Effects of Melatonin and Octreotide Against Radiation-Induced Intestinal Injury [Elektronische Daten] [Cem Onal, Fazilet Kayaselcuk, Erkan Topkan, Melek Yavuz, Didem Bacanli, Aydin Yavuz] Purpose: To compare the protective effects of the potent antioxidants, melatonin and octreotide, against radiation-induced intestinal injury. Methods: A total of 42 male 3-month-old Swiss albino mice (40±10g) were matched according to body weight and randomly assigned to one of six groups: control; radiation treatment (RT) only; melatonin only (15mg/kg, i.p.); melatonin+RT; octreotide only (50μg/kg i.p.); and octreotide+RT. Intestinal damage was induced by exposure to a single whole-body radiation dose of 8Gy. All mice tolerated the experimental interventions, and no deaths were observed. Results: Irradiation induced architectural disorganization, including inflammatory mononuclear cell infiltration, villitis, and desquamation with eosinophilic necrosis, and diminished mucosal thickness, crypt height, and villous height. In the melatonin+RT and octreotide+RT groups, the villous pattern was well preserved; desquamation at villous tips and edema was prominent, but necrosis was absent. The radiation-induced decrease in mucosal thickness was significantly reduced by pretreatment with melatonin (p<0.001) or octreotide (p=0.01), although the protective effect was significantly greater for melatonin (p=0.04). Pretreatment with melatonin also preserved villous height (p=0.009) and crypt height (p=0.03); although a similar trend was observed for pre-irradiation octreotide, the differences were not significant. Conclusions: Melatonin and octreotide potently protected against radiation-induced intestinal injury in mice, but melatonin was significantly more effective in preserving the histological structure of the intestines, a finding that warrants confirmation in clinical studies. Springer Science+Business Media, LLC, 2010 Melatonin nationallicence Octreotide nationallicence Radiation enteritis nationallicence Animal nationallicence Radiotherapy nationallicence Onal Cem Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut Kayaselcuk Fazilet Department of Pathology, Baskent University Medical Faculty, Ankara, Turkey aut Topkan Erkan Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut Yavuz Melek Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut Bacanli Didem Experimental Animals Breeding and Research Center, Baskent University Medical Faculty, Baglica, Ankara, Turkey aut Yavuz Aydin Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 359-367 0163-2116 56:2<359 2011 56 10620 https://doi.org/10.1007/s10620-010-1322-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1322-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Onal Cem Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut NATIONALLICENCE 700 1- Kayaselcuk Fazilet Department of Pathology, Baskent University Medical Faculty, Ankara, Turkey aut NATIONALLICENCE 700 1- Topkan Erkan Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut NATIONALLICENCE 700 1- Yavuz Melek Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut NATIONALLICENCE 700 1- Bacanli Didem Experimental Animals Breeding and Research Center, Baskent University Medical Faculty, Baglica, Ankara, Turkey aut NATIONALLICENCE 700 1- Yavuz Aydin Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 359-367 0163-2116 56:2<359 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer