caa a22 4500 445797592 CHVBK 20180317145130.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10620-010-1316-0 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1316-0 Infection with Specific Helicobacter pylori - cag Pathogenicity Island Strains Is Associated with Interleukin-1B Gene Polymorphisms in Venezuelan Chronic Gastritis Patients [Elektronische Daten] [Miguel Chiurillo, Yeinmy Moran, Miryan Cañas, Elvis Valderrama, Emma Armanie] Background: The cag pathogenicity island (cag-PAI) is one of the major virulence factors of Helicobacter pylori, showing considerable geographic variation. Aim: We investigated the prevalence of cagA, cagE, and cagT genes of cag-PAI and their association with proinflammatory IL-1B-511/-31/+3954 polymorphisms in Venezuelan chronic gastritis patients from a high-risk gastric cancer region. Methods: Presence of cag-PAI genes and IL-1B polymorphisms in 121 biopsy specimens was evaluated by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP), respectively. Results: cagA+ and triple-positive (cagAET+) strains were detected in 79.3% and 70.2% of patients, respectively. We found that infection with cagA+ and cagAET+ strains was associated (P<0.05) with hosts harboring both IL-1B+3954C allele and IL-1B-511T/-31C/+3954C haplotype (TCC+). The frequency of gastric atrophy was significantly higher (P<0.020) among cagAET+/IL-1B-TCC+ combined genotype carriers. Conclusion: Carriage of IL-1B+3954C allele and IL-1B-TCC + haplotype could favor colonization of bacterial cagAET + strains, and the combination of these bacterial and host haplotypes could play a synergistic role in development of premalignant gastric lesions. This work contributes to understanding of the complex interaction between H.pylori virulence factors and cytokine genotypes involved in gastrointestinal diseases. Springer Science+Business Media, LLC, 2010 Helicobacter pylori nationallicence cag pathogenicity island nationallicence Chronic gastritis nationallicence IL-1B polymorphisms nationallicence Chiurillo Miguel Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), 3001, Barquisimeto, Venezuela aut Moran Yeinmy Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), 3001, Barquisimeto, Venezuela aut Cañas Miryan Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), 3001, Barquisimeto, Venezuela aut Valderrama Elvis Departamento de Anatomía Patológica, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela aut Armanie Emma Servicio de Gastroenterología, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 449-456 0163-2116 56:2<449 2011 56 10620 https://doi.org/10.1007/s10620-010-1316-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1316-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chiurillo Miguel Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), 3001, Barquisimeto, Venezuela aut NATIONALLICENCE 700 1- Moran Yeinmy Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), 3001, Barquisimeto, Venezuela aut NATIONALLICENCE 700 1- Cañas Miryan Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), 3001, Barquisimeto, Venezuela aut NATIONALLICENCE 700 1- Valderrama Elvis Departamento de Anatomía Patológica, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela aut NATIONALLICENCE 700 1- Armanie Emma Servicio de Gastroenterología, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 449-456 0163-2116 56:2<449 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer