caa a22 4500 445797630 CHVBK 20180317145131.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10620-010-1285-3 doi (NATIONALLICENCE)springer-10.1007/s10620-010-1285-3 Detection of Differentially Expressed microRNAs in Serum of Pancreatic Ductal Adenocarcinoma Patients: miR-196a Could Be a Potential Marker for Poor Prognosis [Elektronische Daten] [Xiangyu Kong, Yiqi Du, Guokun Wang, Jun Gao, Yanfang Gong, Lei Li, Zhuo Zhang, Jiaqi Zhu, Qing Jing, Yongwen Qin, Zhaoshen Li] Background: MicroRNAs (miRNAs) have long been established to remain stable in circulation, and dysregulated miRNAs in serum of tumor patients could potentially serve as novel biomarkers. Aims: To determine whether certain serum miRNAs could represent potential diagnostic and prognostic biomarkers for pancreatic ductal adenocarcinoma (PDAC). Methods: About 35 patients diagnosed with PDAC at different stages between August 2007 and January 2009 were enrolled in this study. Sera from 15 chronic pancreatitis (CP) patients and 15 healthy individuals were treated as controls. Quantitative real-time polymerase chain reaction assays specific to mature miRNAs were used to quantify the relative levels of those PDAC-associated serum miRNAs. Results: Of the seven miRNAs detected, three were identified as differentially expressed in PDAC and control groups. miR-21 was able to distinguish PDAC patients from CP (p=0.033) and healthy subjects (p=0.001), whereas miR-155 and miR-196a were able to differentiate sera with sick pancreas (PDAC/CP) from normal pancreas (p=0.0002 and 0.010, respectively). Serum miR-196a expression levels in unresectable PDAC (stages III and IV) patients were significantly higher than those in resectable (stages I and II) patients (p=0.001). Furthermore, serum miR-196a expression level was found to have a potential value in predicting median survival time of PDAC patients (high-level miR-196a, 6.1months, (95% CI, 4.49-7.72) versus low-level miR-196a, 12.00months, (95% CI, 5.92-18.08), p=0.007). Conclusions: Serum miR-196a could be a potential noninvasive marker for PDAC prognosis and selection of laparotomy. Springer Science+Business Media, LLC, 2010 Pancreatic cancer nationallicence Serum microRNA nationallicence Biomarker nationallicence miR-196a nationallicence miRNA : microRNA nationallicence PDAC : Pancreatic ductal adenocarcinoma nationallicence CP : Chronic pancreatitis nationallicence ROC : Receiver operating characteristic nationallicence TNM : Tumor-node-metastasis nationallicence Kong Xiangyu Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Du Yiqi Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Wang Guokun Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Gao Jun Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Gong Yanfang Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Li Lei Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Zhang Zhuo Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Zhu Jiaqi Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Jing Qing Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Qin Yongwen Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Li Zhaoshen Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 602-609 0163-2116 56:2<602 2011 56 10620 https://doi.org/10.1007/s10620-010-1285-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-010-1285-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kong Xiangyu Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Du Yiqi Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Wang Guokun Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Gao Jun Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Gong Yanfang Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Li Lei Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Zhuo Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Zhu Jiaqi Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Jing Qing Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Qin Yongwen Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Li Zhaoshen Department of Gastroenterology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/2(2011-02-01), 602-609 0163-2116 56:2<602 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer