caa a22 4500 445797886 CHVBK 20180317145131.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10620-011-1730-y doi (NATIONALLICENCE)springer-10.1007/s10620-011-1730-y Effects of Bifidobacterium infantis 35624 on Post-Inflammatory Visceral Hypersensitivity in the Rat [Elektronische Daten] [Anthony Johnson, Beverley Greenwood-Van Meerveld, John McRorie] Background: Irritable bowel syndrome patients have abnormal visceral perception. Probiotic organisms may produce beneficial effects in these patients by reducing visceral hypersensitivity. Aim: To investigate the effects of the probiotic organism, Bifidobacterium infantis 35624, on post-inflammatory visceral hypersensitivity in rats. Methods: Colitis was induced using intracolonic administration of trinitrobenzenesulfonic acid; control rats received saline (day 0). Myeloperoxidase (MPO) levels and colonic damage scores were determined. From days 15-29, rats (n=10/group) rats were orally dosed with 2ml of B. infantis≥108 colony-forming units/ml or vehicle (MRS broth). A second series of rats (n=10/group) was dosed in the same manner from days 15-59. The level of colonic stimulation during colorectal distension (CRD) was determined by recording a visceromotor response (VMR) to CRD at 30mmHg pre- and post-treatment. Post-treatment samples of colonic tissue were weighed, graded for morphologic damage, and assayed for MPO levels. Results: All rats were hypersensitive at day 15. On day 30, hypersensitivity to colorectal distension remained in the vehicle group, but was significantly reduced in the B. infantis group (mean VMR/10min: vehicle=15.4±1.0 vs. B. infantis=7.6±1.0, p<0.001). A similar, significant effect was observed at day 60. On both day 30 and day 60, tissue weight, colonic damage scores, and MPO levels resembled those of control animals. Conclusions: Oral administration of Bifidobacterium infantis 35624 normalized sensitivity to colorectal distension in a rat model of post-inflammatory colonic hypersensitivity. Springer Science+Business Media, LLC, 2011 Bifidobacterium nationallicence Animal disease model nationallicence Irritable bowel syndrome nationallicence Probiotics nationallicence Rats nationallicence Johnson Anthony Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, 73104, Oklahoma City, OK, USA aut Greenwood-Van Meerveld Beverley Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, 73104, Oklahoma City, OK, USA aut McRorie John The Procter and Gamble Company, 8700 Mason Montgomery Road, 45040, Mason, OH, USA aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/11(2011-11-01), 3179-3186 0163-2116 56:11<3179 2011 56 10620 https://doi.org/10.1007/s10620-011-1730-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1730-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson Anthony Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, 73104, Oklahoma City, OK, USA aut NATIONALLICENCE 700 1- Greenwood-Van Meerveld Beverley Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, 73104, Oklahoma City, OK, USA aut NATIONALLICENCE 700 1- McRorie John The Procter and Gamble Company, 8700 Mason Montgomery Road, 45040, Mason, OH, USA aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/11(2011-11-01), 3179-3186 0163-2116 56:11<3179 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer