caa a22 4500 445798041 CHVBK 20180317145132.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10620-011-1747-2 doi (NATIONALLICENCE)springer-10.1007/s10620-011-1747-2 Lactobacillus plantarum Prevents Bacterial Translocation in Rats Following Ischemia and Reperfusion Injury [Elektronische Daten] [Bin Wang, Qian Huang, Wei Zhang, Ning Li, Jieshou Li] Background: Bacterial translocation is considered a major cause of initiation and development of systemic sepsis and multiple organ dysfunction in clinic. The aim of this study was to determine the ability of a defined Lactobacillus plantarum to prevent ischemia/reperfusion (I/R) induced intestinal infection. Methods: Female Sprague-Dawley rats were randomly allocated into three groups: (1) controls (sham-operated, no treatment), (2) ischemia/reperfusion and (3) ischemia/reperfusion and Lactobacillus plantarum treatment. Lactobacillus plantarum L2 was administered daily intragastrically 14days prior to induction of I/R. Rats were then sacrificed, and tissue and blood samples were cultured to determine bacterial translocation. Cytokines in plasma were detected by ELISA. Ileal segments were removed for morphological examination. Results: Intestinal I/R induced excess pro-inflammatory cytokine secretion and barrier dysfunction (increased epithelial cell apoptosis, cecal flora dysbiosis, disruption of mucosa and multiple erosions) in the intestine, associated with increased bacterial translocation to extraintestinal sites. Approximately 87.5% of rats exposed to I/R had bacterial translocation while there was no bacterial translocation in controls. However, pretreatment of animals with Lactobacillus plantarum completely prevented I/R induced bacterial translocation, reduced pro-inflammatory cytokine release, and intestinal epithelial cell apoptosis, resulting in recovered microflora and mucosal integrity. Conclusions: These findings indicate that Lactobacillus plantarum L2 can prevent I/R-induced bacterial translocation and intestinal barrier dysfunction and, thereby, exert beneficial effects in the intestinal tract. Springer Science+Business Media, LLC, 2011 Lactobacillus plantarum nationallicence Ischemia and reperfusion nationallicence Bacterial translocation nationallicence Wang Bin Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut Huang Qian Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut Zhang Wei Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut Li Ning Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut Li Jieshou Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/11(2011-11-01), 3187-3194 0163-2116 56:11<3187 2011 56 10620 https://doi.org/10.1007/s10620-011-1747-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10620-011-1747-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Bin Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Huang Qian Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Wei Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Li Ning Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Li Jieshou Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, 210002, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Digestive Diseases and Sciences Springer US; http://www.springer-ny.com 56/11(2011-11-01), 3187-3194 0163-2116 56:11<3187 2011 56 10620 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer