caa a22 4500 445801042 CHVBK 20180317145141.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1899-9 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1899-9 Separation of Steroids and the Determination of Estradiol Content in Transdermic Patches by Microemulsion Electrokinetic Chromatography [Elektronische Daten] [Claudinei Silva, María Aurora-Prado, Kevin Altria, Marina Tavares] A novel microemulsion electrokinetic capillary chromatography (MEEKC) method has been developed which separates a range of nine steroids. A microemulsion containing ethyl acetate, butan-1-ol, sodium dodecyl sulfate, 15% (v/v) acetonitrile and 12mmol L−1 sodium tetraborate aqueous buffer at pH 9.2 was used with direct UV detection at 200nm. The method was validated for the determination of 17β-estradiol content, a hormone steroid, in transdermal patches. Adequate sensitivity (DL=0.88μgmL−1; QL=2.65μgmL−1) without interference from sample excipients was obtained. 17β-Estradiol migrates in approximately 5.4min. Estrone was used as internal standard and acceptable precision (<1.2% RSD), linearity (r=0.9996; range from 40.0 to 60.0μgmL−1), and recovery (100.4±0.9% at three concentration levels) were obtained. The principal advantage of the method is that it is rapid and avoids the need of time consuming and expensive sample pre-treatment steps. Graphical Abstract: Springer-Verlag, 2011 Microemulsion electrokinetic chromatography nationallicence Steroids nationallicence 17β-Estradiol nationallicence Transdermal patches nationallicence Silva Claudinei Institute of Chemistry, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, 05508-900, Sao Paulo, SP, Brazil aut Aurora-Prado María Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580, 05508-900, Sao Paulo, SP, Brazil aut Altria Kevin GlaxoSmithKline Pharmaceutical Development, CM19 5AW, Harlow, Essex, UK aut Tavares Marina Institute of Chemistry, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, 05508-900, Sao Paulo, SP, Brazil aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 373-378 0009-5893 73:3-4<373 2011 73 10337 https://doi.org/10.1007/s10337-010-1899-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1899-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Silva Claudinei Institute of Chemistry, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, 05508-900, Sao Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Aurora-Prado María Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580, 05508-900, Sao Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Altria Kevin GlaxoSmithKline Pharmaceutical Development, CM19 5AW, Harlow, Essex, UK aut NATIONALLICENCE 700 1- Tavares Marina Institute of Chemistry, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, 05508-900, Sao Paulo, SP, Brazil aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 373-378 0009-5893 73:3-4<373 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer