caa a22 4500 445801158 CHVBK 20180317145141.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1896-z doi (NATIONALLICENCE)springer-10.1007/s10337-010-1896-z Comparison of UPLC and HPLC for Analysis of Dietary Folates [Elektronische Daten] [Johan Jastrebova, Hanna Strandler, Jelena Patring, Tony Wiklund] Ultra performance liquid chromatography (UPLC) using small sub-2μm particles and high performance liquid chromatography (HPLC) were compared for separation and determination of the most common dietary folates; 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, 10-formylfolic acid and folic acid. Two UPLC columns—Acquity BEH C18 and Acquity HSS T3, and two HPLC columns with similar surface chemistry—Xbridge C18 and Atlantis d18 were tested. When using UPLC, the signal-to-noise ratio could be improved by a factor of 2-50 for different folate derivatives and the run time could be reduced fourfold without sacrificing separation efficiency. The applicability of UPLC to real food samples was demonstrated. Springer-Verlag, 2011 Ultra performance liquid chromatography nationallicence Ultra fast separation nationallicence Sub-2μm particles nationallicence Folate food analysis nationallicence Jastrebova Johan Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden aut Strandler Hanna Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden aut Patring Jelena Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden aut Wiklund Tony Waters Sweden, Box 485, 191 24, Sollentuna, Sweden aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 219-225 0009-5893 73:3-4<219 2011 73 10337 https://doi.org/10.1007/s10337-010-1896-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1896-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jastrebova Johan Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Strandler Hanna Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Patring Jelena Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Wiklund Tony Waters Sweden, Box 485, 191 24, Sollentuna, Sweden aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 219-225 0009-5893 73:3-4<219 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer