caa a22 4500 445801166 CHVBK 20180317145141.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1891-4 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1891-4 Chiral Separation of β-Adrenergic Blockers in Human Plasma by SPE-HPLC [Elektronische Daten] [Imran Ali, Zeid Al-Othman, Afzal Hussain, Kishwar Saleem, Hassan Aboul-Enein] Chiral analysis of β-adrenergic blockers in human plasma is an important research area due to their different pharmaceutical activities. The solid phase extraction of alprenolol, carazolol, metoprolol, oxprenolol from human plasma was achieved on C18 cartridges using phosphate buffer (50mM, pH 9.0) followed by elution with methanol containing 0.1% acetic acid. Chiral-HPLC was carried out on CelluCoat (250×4.6mm, 5.0μm silica particle size) column using different combinations of n-heptane-ethanol-diethylamine at 1.0 and 2.0 mLmin−1 flow rates, respectively. The detection was achieved at 225nm with 27±1°C as working temperature. The chromatographic parameters, i.e., retention (k), separation (α) and resolution (Rs) factors ranged from 0.26 to 10.64, 1.12 to 2.10 and 1.05 to 2.26, respectively. The binding differences of enantiomeric concentrations of oxprenolol, carazolol, alprenolol, and metoprolol were 0.12, 0.08, 0.05, and 0.01, respectively. These values suggest that their activities may be in the order of oxprenolol>carazolol>alprenolol>metoprolol. The reported SPE-Chiral HPLC methods may be used to study the enantiomeric concentrations of these β-adrenergic blockers in human and other animal plasmas for further studies. Springer-Verlag, 2011 β-Adrenergic blockers nationallicence Column liquid chromatography nationallicence Chiral separation nationallicence Human plasma nationallicence Plasma protein bindings nationallicence SPE-chiral HPLC nationallicence Solid phase extraction nationallicence Ali Imran Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, 110025, New Delhi, India aut Al-Othman Zeid Department of Chemistry, College of Science, King Saud University, P.O. Box No. 2455, 11451, Riyadh, Kingdom of Saudi Arabia aut Hussain Afzal Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, 110025, New Delhi, India aut Saleem Kishwar Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, 110025, New Delhi, India aut Aboul-Enein Hassan Pharmaceutical and Drug Industries Research Division, Pharmaceutical and Medicinal Chemistry Department, National Reasearch Centre, Dokki, 12311, Cairo, Egypt aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 251-256 0009-5893 73:3-4<251 2011 73 10337 https://doi.org/10.1007/s10337-010-1891-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1891-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ali Imran Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Al-Othman Zeid Department of Chemistry, College of Science, King Saud University, P.O. Box No. 2455, 11451, Riyadh, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Hussain Afzal Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Saleem Kishwar Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Aboul-Enein Hassan Pharmaceutical and Drug Industries Research Division, Pharmaceutical and Medicinal Chemistry Department, National Reasearch Centre, Dokki, 12311, Cairo, Egypt aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 251-256 0009-5893 73:3-4<251 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer