caa a22 4500 445801212 CHVBK 20180317145141.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1893-2 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1893-2 Semi-Preparative Isolation and Purification of Three Tauro-Conjugated Cholic Acids from Pulvis Fellis Suis by HSCCC Coupled with ELSD Detection [Elektronische Daten] [Jiao He, Yongmin Zhang, Yoichiro Ito, Wenji Sun] Coupled with evaporative light scattering detection, a high-speed countercurrent chromatography method was successfully applied to the separation and purification of three tauro-conjugated cholic acids from the traditional Chinese medicine Pulvis Fellis Suis. With a two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v), taurochenodeoxycholic acid (10.2mg), taurohyodeoxycholic acid (11.8mg) and taurohyocholic acid (5.3mg), were obtained from 100mg of the crude extract with purities of 94.6, 96.5 and 95.4%, respectively, in one-step separation. The structures of the three tauro-conjugated cholic acids were identified by ESI-MS, 1H NMR and 13C NMR. Springer-Verlag, 2011 High-speed countercurrent chromatography nationallicence Tauro-conjugated cholic acids nationallicence Pulvis Fellis Suis nationallicence Sus scrofa domestica Brisson nationallicence He Jiao Biomedicine Key Laboratory of Shaanxi Province, Northwest University, No. 229 Taibai North Road, 710069, Xi'an, People's Republic of China aut Zhang Yongmin Institut Parisien de Chimie Moléculaire, Université Pierre and Marie Curie-Paris 6 (UMR CNRS 7201), 4 place Jussieu, 75005, Paris, France aut Ito Yoichiro Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA aut Sun Wenji Biomedicine Key Laboratory of Shaanxi Province, Northwest University, No. 229 Taibai North Road, 710069, Xi'an, People's Republic of China aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 361-365 0009-5893 73:3-4<361 2011 73 10337 https://doi.org/10.1007/s10337-010-1893-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1893-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Jiao Biomedicine Key Laboratory of Shaanxi Province, Northwest University, No. 229 Taibai North Road, 710069, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yongmin Institut Parisien de Chimie Moléculaire, Université Pierre and Marie Curie-Paris 6 (UMR CNRS 7201), 4 place Jussieu, 75005, Paris, France aut NATIONALLICENCE 700 1- Ito Yoichiro Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Sun Wenji Biomedicine Key Laboratory of Shaanxi Province, Northwest University, No. 229 Taibai North Road, 710069, Xi'an, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 361-365 0009-5893 73:3-4<361 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer