caa a22 4500 445801220 CHVBK 20180317145141.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1844-y doi (NATIONALLICENCE)springer-10.1007/s10337-010-1844-y Determination of Nerve Growth Factor in Rat Spinal Cord by Capillary Electrophoresis-Based Immunoassay with a Laser Induced Fluorescence Detector [Elektronische Daten] [Xiaoli Zou, Yuanqian Li, Tinghua Wang, Chunyan Zhou, Hongyan Zeng] In order to study the relationship between spinal cord injury and the change of nerve growth factor (NGF), an analytical method for NGF by capillary electrophoresis-based immunoassay (CEIA) with laser-induced fluorescence (LIF) was developed. Having been dissolved in phosphate buffer solution and concentrated with vacuum freeze-drying, NGF in spinal cord of rat was allowed to react with NGF monoclonal antibody labeled with fluorescence isothiocyanate (FITC). Then the immuno-complex, FITC-labeled anti-NGF and FITC were separated and determined by LIF-CEIA using Kiton red as the internal standard. The linear range of the method was 2-30ngmL−1 and the limit of detection was 0.35ngmL−1. The relative standard derivations for relative migration time and relative fluorescence intensity ratio were 7.98% and 6.52%, respectively. The contents of NGF from spinal cord of rat were determined by both the proposed method and Western blotting. The results with the two methods agreed well. The spiked recoveries of the samples were 88.5-116.3%. The proposed method was rapid, precise and inexpensive. Springer-Verlag, 2011 Capillary electrophoresis-based immunoassay nationallicence Laser-induced fluorescence nationallicence Nerve growth factor nationallicence Spinal cord of rat nationallicence Zou Xiaoli Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut Li Yuanqian Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut Wang Tinghua Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut Zhou Chunyan Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut Zeng Hongyan Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 307-312 0009-5893 73:3-4<307 2011 73 10337 https://doi.org/10.1007/s10337-010-1844-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1844-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zou Xiaoli Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Li Yuanqian Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Tinghua Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Chunyan Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Zeng Hongyan Department of Sanitary Technology, West China School of Public Health, Sichuan University, No.16 Renming South Road, 610041, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 307-312 0009-5893 73:3-4<307 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer