caa a22 4500 445801263 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1898-x doi (NATIONALLICENCE)springer-10.1007/s10337-010-1898-x Reversed-Phase Ion-Pair HPLC and TLC-Densitometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets [Elektronische Daten] [Ehab Elkady, Marianne Mahrouse] Two methods are described for the simultaneous determination of ciprofloxacin HCl (CIP) and metronidazole (MET) in binary mixture. The first method was based on the ion-pair liquid chromatographic separation of the two drugs on reversed-phase, μBondapak C18 column (250mm×4.6mm, 10μm) Waters. The mobile phase consisted of monobasic potassium phosphate (50mM, pH 3, adjusted with phosphoric acid) and acetonitrile (65:35, v/v) containing sodium octane sulphonate (50mM). Flow rate of 1.2mLmin−1 was applied. Quantitation was achieved with UV detection at 280nm. Linearity, accuracy and precision were found to be acceptable over the concentration range of 3-180 and 15-180μgmL−1 for CIP and MET, respectively. The second method was based on the TLC separation of the two drugs followed by densitometric measurements of their bands at 280nm. The separation was carried out on silica gel 60 F254 plates, using acetonitrile, ammonia, methanol, methylene chloride and hexane (1.3:1.1:2.0:3.0:1.0, v/v) as developing system. The linear regression analysis data were used for the regression line in the range of 1.5-10μg band−1 for CIP and MET. The two proposed methods were successfully applied to the determination of both drugs in laboratory prepared mixtures and in commercial tablets. The optimized methods proved to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical preparation. Springer-Verlag, 2011 Reversed-phase ion-pair liquid chromatography nationallicence TLC densitometry nationallicence Antibacterial drugs nationallicence Ciprofloxacin hydrochloride nationallicence Metronidazole nationallicence Elkady Ehab Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St, 11562, Cairo, Egypt aut Mahrouse Marianne Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St, 11562, Cairo, Egypt aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 297-305 0009-5893 73:3-4<297 2011 73 10337 https://doi.org/10.1007/s10337-010-1898-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1898-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Elkady Ehab Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St, 11562, Cairo, Egypt aut NATIONALLICENCE 700 1- Mahrouse Marianne Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St, 11562, Cairo, Egypt aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 297-305 0009-5893 73:3-4<297 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer