caa a22 4500 445801271 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1876-3 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1876-3 HPLC Separation of Nimesulide and Five Impurities using a Narrow-Bore Monolithic Column: Application to Photo-Degradation Studies [Elektronische Daten] [Constantinos Zacharis, Paraskevas Tzanavaras] Nimesulide and its fine impurities were separated using the new narrow-bore FastGradient monolithic column (50×2.0mm i.d.). The mobile phase was a mixture of NH4H2PO4 (10mmolL−1, pH 7)/ACN (69:31 v/v) and UV detection was carried out at 230nm. Depending on the flow rate of the mobile phase (0.5-1.0mLmin−1), efficient separation of the six compounds could be achieved within 5-10min. The developed HPLC method was validated in terms of linearity, limits of detection and quantitation, precision, selectivity, and accuracy. Application to the photo-stability of nimesulide revealed a ca. 50% degradation of the active pharmaceutical ingredient within 24h. Impurity D was also identified at a maximum level of 0.46% (24h). Springer-Verlag, 2011 Column liquid chromatography nationallicence FastGradient monolithic column nationallicence Photochemical stability nationallicence Nimesulide nationallicence Zacharis Constantinos Laboratory of Analytical Chemistry, Department of Chemistry, Aristotelian University of Thessaloniki, 54124, Thessaloniki, Greece aut Tzanavaras Paraskevas Laboratory of Analytical Chemistry, Department of Chemistry, Aristotelian University of Thessaloniki, 54124, Thessaloniki, Greece aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 347-352 0009-5893 73:3-4<347 2011 73 10337 https://doi.org/10.1007/s10337-010-1876-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1876-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zacharis Constantinos Laboratory of Analytical Chemistry, Department of Chemistry, Aristotelian University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Tzanavaras Paraskevas Laboratory of Analytical Chemistry, Department of Chemistry, Aristotelian University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 347-352 0009-5893 73:3-4<347 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer