caa a22 4500 445801298 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1867-4 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1867-4 Quantitative Determination of Phillyrin in Human Plasma Using HPLC with Fluorescence Detection [Elektronische Daten] [Zhenyuan Zhang, Jingdong Peng] A simple, rapid, and selective high-performance liquid chromatography method for determination of phillyrin in human plasma was developed. After extracting from the plasma samples with ethyl acetate, the analyte was chromatographed on a C18 column with methanol-water (50:50, v/v, pH2.86) as mobile phase. The fluorescence excitation and emission wavelengths were 277 and 315nm, respectively. The linear range of the standard curve of phillyrin was 0.0313-8.0μgmL−1 (r>0.999). The limit of detection was 6.31ngmL−1. The average recovery of phillyrin was 101.02% from plasma. The intra- and inter-day variabilities of phillyrin were <10.00%. Springer-Verlag, 2011 Column liquid chromatography nationallicence Fluorescence detection nationallicence Human plasma nationallicence Phillyrin nationallicence Zhang Zhenyuan School of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, China aut Peng Jingdong School of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, China aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 415-417 0009-5893 73:3-4<415 2011 73 10337 https://doi.org/10.1007/s10337-010-1867-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1867-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Zhenyuan School of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, China aut NATIONALLICENCE 700 1- Peng Jingdong School of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 415-417 0009-5893 73:3-4<415 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer